Beta-galactosidase assay

From OpenWetWare

Methods to assay for beta-galactosidase

Specific protocols

• Media:B-gal protocol.doc
• Sauer:beta-Galactosidase Assay (A better Miller) <-- highly recommend
• BE.109:Protein engineering/Assessing beta-galactosidase
• Knight:Beta-galactosidase_assay
• Knight:Beta-galactosidase assay/96 well format
• Knight:Beta-galactosidase assay/Fluorescence
• See also Beta-galactosidase assays in yeast

See also

• Beta-galactosidase Screen
• LacZ staining of cells

References

1. [by] Jeffrey H. Miller. Experiments in molecular genetics. [Cold Spring Harbor, N.Y.] Cold Spring Harbor Laboratory, 1972. isbn:0879691069. [Miller-1972]

   original β-galactosidase assay by Miller

2. Griffith KL and Wolf RE Jr. . pmid:11779182. PubMed HubMed [Griffith-2002]

   96 well format