Berkmen - Revision history

Melanie berkmen: /* Current Members of the Berkmen Lab */

Melanie berkmen — Mon, 23 Jul 2012 18:40:26 GMT

Current Members of the Berkmen Lab

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-	'''Azul Pinochet Barros''' - ~~Philosophy/~~Biology Major<br>	+	'''Azul Pinochet Barros''' - Biology/Philosophy Major<br>
	Azul joined the lab in the spring of 2012. With an interest in cyanobacteria, Azul was very determined to be involved in bacterial growth, so she has helped optimize our competent cells procedure. <br>		Azul joined the lab in the spring of 2012. With an interest in cyanobacteria, Azul was very determined to be involved in bacterial growth, so she has helped optimize our competent cells procedure. <br>
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Melanie berkmen: /* Publications */

Melanie berkmen — Mon, 23 Jul 2012 18:23:35 GMT

Publications

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	== Publications ==		== Publications ==
-	[[Suffolk undergraduate authors in red	+	[[Suffolk undergraduate authors in red]]
-	]]<br>	+	<br>
	<br>		<br>
	'''Berkmen MB''', [[Laurer SJ, Giarusso BK, and Romero R]]. (2012)The Integrative and Conjugative Element ICE''Bs1'' of ''Bacillus subtilis''. Review chapter in the book Bacterial Integrative Mobile Genetic Elements, edited by Adam P. Roberts and Peter Mullany, Landes Bioscience.		'''Berkmen MB''', [[Laurer SJ, Giarusso BK, and Romero R]]. (2012)The Integrative and Conjugative Element ICE''Bs1'' of ''Bacillus subtilis''. Review chapter in the book Bacterial Integrative Mobile Genetic Elements, edited by Adam P. Roberts and Peter Mullany, Landes Bioscience.

Melanie berkmen: /* Former Members of the Berkmen Lab */

Melanie berkmen — Mon, 23 Jul 2012 18:23:09 GMT

Former Members of the Berkmen Lab

←Older revision		Revision as of 18:23, 23 July 2012
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	== Former Members of the Berkmen Lab ==		== Former Members of the Berkmen Lab ==

-	'''Stephanie Laurer''' - B.S. Biochemistry, Honors Program, May 2012<br>	+	'''Stephanie Laurer''' - B.A. Biochemistry, Honors Program, May 2012<br>
	Stephanie used mating assays to determine that addition of a His6-tag on the N-terminus of ConE does not interfere with ConE's ability to support mating. She presented this research with Bridget at the 2010 ACS National Meeting in San Francisco and at the Suffolk Science Banquet where they won a poster award. She also helped clone several ICE''Bs''1 genes. On the side, Stephanie had an interest in genetically modified food. During her first year at Suffolk, she used a PCR-based assay to detect the Bt gene in corn. She found that at least half of the samples she tested (6 of 11) were genetically modified. She presented this work at the 2009 Suffolk science banquet where her poster won first place. She now acts as Clinical Research Assistant at Beth Israel Deaconess Medical Center.<br>		Stephanie used mating assays to determine that addition of a His6-tag on the N-terminus of ConE does not interfere with ConE's ability to support mating. She presented this research with Bridget at the 2010 ACS National Meeting in San Francisco and at the Suffolk Science Banquet where they won a poster award. She also helped clone several ICE''Bs''1 genes. On the side, Stephanie had an interest in genetically modified food. During her first year at Suffolk, she used a PCR-based assay to detect the Bt gene in corn. She found that at least half of the samples she tested (6 of 11) were genetically modified. She presented this work at the 2009 Suffolk science banquet where her poster won first place. She now acts as Clinical Research Assistant at Beth Israel Deaconess Medical Center.<br>
	<br>		<br>

Melanie berkmen: /* Former Members of the Berkmen Lab */

Melanie berkmen — Mon, 23 Jul 2012 18:21:21 GMT

Former Members of the Berkmen Lab

Melanie berkmen: /* Publications */

Melanie berkmen — Mon, 23 Jul 2012 18:17:44 GMT

Publications

←Older revision		Revision as of 18:17, 23 July 2012
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	== Publications ==		== Publications ==
-	Suffolk undergraduate authors ~~underlined~~	+	[[Suffolk undergraduate authors in red
-	<br>	+	]]<br>
	<br>		<br>
	'''Berkmen MB''', [[Laurer SJ, Giarusso BK, and Romero R]]. (2012)The Integrative and Conjugative Element ICE''Bs1'' of ''Bacillus subtilis''. Review chapter in the book Bacterial Integrative Mobile Genetic Elements, edited by Adam P. Roberts and Peter Mullany, Landes Bioscience.		'''Berkmen MB''', [[Laurer SJ, Giarusso BK, and Romero R]]. (2012)The Integrative and Conjugative Element ICE''Bs1'' of ''Bacillus subtilis''. Review chapter in the book Bacterial Integrative Mobile Genetic Elements, edited by Adam P. Roberts and Peter Mullany, Landes Bioscience.

Melanie berkmen: /* Publications */

Melanie berkmen — Mon, 23 Jul 2012 18:14:00 GMT

Publications

←Older revision		Revision as of 18:14, 23 July 2012
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	== Publications ==		== Publications ==
-		+	Suffolk undergraduate authors underlined
-	'''Berkmen MB''', Laurer SJ, Giarusso BK, and Romero R. (2012)The Integrative and Conjugative Element ICE''Bs1'' of ''Bacillus subtilis''. Review chapter in the book Bacterial Integrative Mobile Genetic Elements, edited by Adam P. Roberts and Peter Mullany, Landes Bioscience.	+	<br>
		+	<br>
		+	'''Berkmen MB''', [[Laurer SJ, Giarusso BK, and Romero R]]. (2012)The Integrative and Conjugative Element ICE''Bs1'' of ''Bacillus subtilis''. Review chapter in the book Bacterial Integrative Mobile Genetic Elements, edited by Adam P. Roberts and Peter Mullany, Landes Bioscience.
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-	'''Berkmen MB''', Lee CA, Loveday EK, Grossman AD. (2010) Polar positioning of a conjugation protein from the integrative and conjugative element ICE''Bs1'' of ''Bacillus subtilis''. J Bacteriol, 192(1):38-45.	+	'''Berkmen MB''', Lee CA, [[Loveday EK]], Grossman AD. (2010) Polar positioning of a conjugation protein from the integrative and conjugative element ICE''Bs1'' of ''Bacillus subtilis''. J Bacteriol, 192(1):38-45.
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	<br>		<br>

Melanie berkmen: /* Publications */

Melanie berkmen — Mon, 23 Jul 2012 18:12:02 GMT

Publications

←Older revision		Revision as of 18:12, 23 July 2012
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	'''Berkmen MB''', Laurer SJ, Giarusso BK, and Romero R. (2012)The Integrative and Conjugative Element ICE''Bs1'' of ''Bacillus subtilis''. Review chapter in the book Bacterial Integrative Mobile Genetic Elements, edited by Adam P. Roberts and Peter Mullany, Landes Bioscience.		'''Berkmen MB''', Laurer SJ, Giarusso BK, and Romero R. (2012)The Integrative and Conjugative Element ICE''Bs1'' of ''Bacillus subtilis''. Review chapter in the book Bacterial Integrative Mobile Genetic Elements, edited by Adam P. Roberts and Peter Mullany, Landes Bioscience.
-		+	<br>
		+	<br>
	Martinez KA 2nd, Kitko RD, Mershon JP, Adcox HE, Malek KA, '''Berkmen MB''', Slonczewski JL. (2012) Cytoplasmic pH response to acid stress in individual cells of ''Escherichia coli'' and ''Bacillus subtilis'' observed by fluorescence ratio imaging microscopy. Appl Environ Microbiol., 78(10):3706-14.		Martinez KA 2nd, Kitko RD, Mershon JP, Adcox HE, Malek KA, '''Berkmen MB''', Slonczewski JL. (2012) Cytoplasmic pH response to acid stress in individual cells of ''Escherichia coli'' and ''Bacillus subtilis'' observed by fluorescence ratio imaging microscopy. Appl Environ Microbiol., 78(10):3706-14.
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Melanie berkmen: /* Publications */

Melanie berkmen — Mon, 23 Jul 2012 18:11:36 GMT

Publications

←Older revision		Revision as of 18:11, 23 July 2012
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	== Publications ==		== Publications ==
		+
		+	'''Berkmen MB''', Laurer SJ, Giarusso BK, and Romero R. (2012)The Integrative and Conjugative Element ICE''Bs1'' of ''Bacillus subtilis''. Review chapter in the book Bacterial Integrative Mobile Genetic Elements, edited by Adam P. Roberts and Peter Mullany, Landes Bioscience.
		+
		+	Martinez KA 2nd, Kitko RD, Mershon JP, Adcox HE, Malek KA, '''Berkmen MB''', Slonczewski JL. (2012) Cytoplasmic pH response to acid stress in individual cells of ''Escherichia coli'' and ''Bacillus subtilis'' observed by fluorescence ratio imaging microscopy. Appl Environ Microbiol., 78(10):3706-14.
		+	<br>
		+	<br>
		+
		+	Babic A, '''Berkmen MB''', Lee CA, Grossman AD. (2011) Efficient gene transfer in bacterial cell chains. MBio, 2(2). pii: e00027-11. doi: 10.1128/mBio.00027-11.
		+	<br>
		+	<br>
	'''Berkmen MB''', Lee CA, Loveday EK, Grossman AD. (2010) Polar positioning of a conjugation protein from the integrative and conjugative element ICE''Bs1'' of ''Bacillus subtilis''. J Bacteriol, 192(1):38-45.		'''Berkmen MB''', Lee CA, Loveday EK, Grossman AD. (2010) Polar positioning of a conjugation protein from the integrative and conjugative element ICE''Bs1'' of ''Bacillus subtilis''. J Bacteriol, 192(1):38-45.
	<br>		<br>

Melanie berkmen: /* Current Members of the Berkmen Lab */

Melanie berkmen — Mon, 23 Jul 2012 17:59:42 GMT

Current Members of the Berkmen Lab

←Older revision		Revision as of 17:59, 23 July 2012
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	== Current Members of the Berkmen Lab ==		== Current Members of the Berkmen Lab ==
-	[[Image:533393 10150880300287619 646892850 n.jpg\|300px\|thumb\|Melanie Berkmen with research assistants Kyle Swerdlow, Stephanie Laurer, Gianna Mancuso, and Azul Pinochet Barros.\|Melanie Berkmen with research assistants Stephanie Laurer, Gianna Mancuso~~, Kyle Swerdlow~~, and Azul Pinochet Barros]]	+	[[Image:533393 10150880300287619 646892850 n.jpg\|300px\|thumb\|Melanie Berkmen with research assistants Kyle Swerdlow, Stephanie Laurer, Gianna Mancuso, and Azul Pinochet Barros.\|Melanie Berkmen with research assistants Kyle Swerdlow, Stephanie Laurer, Gianna Mancuso, and Azul Pinochet Barros]]

	'''Matt Broulidakis''' - Biochemistry Major, Honors Program<br>		'''Matt Broulidakis''' - Biochemistry Major, Honors Program<br>

Melanie berkmen: /* Former Members of the Berkmen Lab */

Melanie berkmen — Mon, 23 Jul 2012 17:59:02 GMT

Former Members of the Berkmen Lab

←Older revision		Revision as of 17:59, 23 July 2012
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	'''Stephanie Laurer''' - B.S. Biochemistry, May 2012<br>		'''Stephanie Laurer''' - B.S. Biochemistry, May 2012<br>
-	~~As a research assistant,~~ Stephanie used mating assays to determine that addition of a His6-tag on the N-terminus of ConE does not interfere with ConE's ability to support mating. She presented this research with Bridget at the 2010 ACS National Meeting in San Francisco and at the Suffolk Science Banquet where they won a poster award. She also helped clone several ICE''Bs''1 genes. On the side, Stephanie had an interest in genetically modified food. During her first year at Suffolk, she used a PCR-based assay to detect the Bt gene in corn. She found that at least half of the samples she tested (6 of 11) were genetically modified. She presented this work at the 2009 Suffolk science banquet where her poster won first place. She now acts as Clinical ~~Coordinating~~ Assistant ~~in the Oncology department~~ at ~~Mass General Hospital~~.<br>	+	Stephanie used mating assays to determine that addition of a His6-tag on the N-terminus of ConE does not interfere with ConE's ability to support mating. She presented this research with Bridget at the 2010 ACS National Meeting in San Francisco and at the Suffolk Science Banquet where they won a poster award. She also helped clone several ICE''Bs''1 genes. On the side, Stephanie had an interest in genetically modified food. During her first year at Suffolk, she used a PCR-based assay to detect the Bt gene in corn. She found that at least half of the samples she tested (6 of 11) were genetically modified. She presented this work at the 2009 Suffolk science banquet where her poster won first place. She now acts as Clinical Research Assistant at Beth Israel Deaconess Medical Center.<br>
	<br>		<br>

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	'''Erin Cross''' - B.S. Biochemistry, May 2009<br>		'''Erin Cross''' - B.S. Biochemistry, May 2009<br>
-	In CHEM L333, Erin and her class mates attempted to clone various C-terminal truncations of YddE fused to GFP. For CHEM L428/L429, she used fluorescence microscopy to analyze what parts of YddE are required for localization to the cell poles. She found that the C-terminal half of YddE is critical for localization. In addition, she found the ~~YddE~~-GFP localizes at the cell poles, even when the upstream gene ''yddD'' is not expressed ''in cis''. She presented this work at the national ACS meeting in March 2009 and at the 2009 Suffolk Science Banquet where her poster won 3rd place. Erin is now working as a laboratory research ~~technicial~~.<br>	+	In CHEM L333, Erin and her class mates attempted to clone various C-terminal truncations of YddE fused to GFP. For CHEM L428/L429, she used fluorescence microscopy to analyze what parts of YddE are required for localization to the cell poles. She found that the C-terminal half of YddE is critical for localization. In addition, she found the ConE-GFP localizes at the cell poles, even when the upstream gene ''yddD'' is not expressed ''in cis''. She presented this work at the national ACS meeting in March 2009 and at the 2009 Suffolk Science Banquet where her poster won 3rd place. Erin is now working as a laboratory research technologist at MMCRI.<br>
	<br>		<br>
	'''Maria Muccioli (formerly Levicheva)''' - B.S. Biochemistry, Honors Program, May 2009<br>		'''Maria Muccioli (formerly Levicheva)''' - B.S. Biochemistry, Honors Program, May 2009<br>

←Older revision		Revision as of 18:21, 23 July 2012
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	== Former Members of the Berkmen Lab ==		== Former Members of the Berkmen Lab ==

-	'''Stephanie Laurer''' - B.S. Biochemistry, May 2012<br>	+	'''Stephanie Laurer''' - B.S. Biochemistry, Honors Program, May 2012<br>
	Stephanie used mating assays to determine that addition of a His6-tag on the N-terminus of ConE does not interfere with ConE's ability to support mating. She presented this research with Bridget at the 2010 ACS National Meeting in San Francisco and at the Suffolk Science Banquet where they won a poster award. She also helped clone several ICE''Bs''1 genes. On the side, Stephanie had an interest in genetically modified food. During her first year at Suffolk, she used a PCR-based assay to detect the Bt gene in corn. She found that at least half of the samples she tested (6 of 11) were genetically modified. She presented this work at the 2009 Suffolk science banquet where her poster won first place. She now acts as Clinical Research Assistant at Beth Israel Deaconess Medical Center.<br>		Stephanie used mating assays to determine that addition of a His6-tag on the N-terminus of ConE does not interfere with ConE's ability to support mating. She presented this research with Bridget at the 2010 ACS National Meeting in San Francisco and at the Suffolk Science Banquet where they won a poster award. She also helped clone several ICE''Bs''1 genes. On the side, Stephanie had an interest in genetically modified food. During her first year at Suffolk, she used a PCR-based assay to detect the Bt gene in corn. She found that at least half of the samples she tested (6 of 11) were genetically modified. She presented this work at the 2009 Suffolk science banquet where her poster won first place. She now acts as Clinical Research Assistant at Beth Israel Deaconess Medical Center.<br>
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	'''Matt Hamada''' - B.S. Biochemistry, December 2010<br>		'''Matt Hamada''' - B.S. Biochemistry, December 2010<br>
-	For CHEM L333, Matt cloned the ''~~yddC~~'' gene encoded on the ICE''Bs1'' conjugative element. For CHEM L428/L429, Matt used fluorescence microscopy to determine whether ''~~yddC~~'' and other ICE''Bs1'' genes are required for ConE to localize at the cell poles. He presented his research with Cori at the 2009 Boston Bacterial Meeting. Currently, Matt is working as a laboratory research technician.<br>	+	For CHEM L333, Matt cloned the ''conC'' gene encoded on the ICE''Bs1'' conjugative element. For CHEM L428/L429, Matt used fluorescence microscopy to determine whether ''conC'' and other ICE''Bs1'' genes are required for ConE to localize at the cell poles. He presented his research with Cori at the 2009 Boston Bacterial Meeting. Currently, Matt is working as a laboratory research technician.<br>
	<br>		<br>

	'''Cori Leonetti''' - B.S. Biochemistry, May 2010<br>		'''Cori Leonetti''' - B.S. Biochemistry, May 2010<br>
-	For CHEM L333, Cori helped clone the ''~~yddB~~'' gene encoded on the ICE''Bs1'' conjugative element. Cori extended her CHEM L333 project for CHEM L428/L429. She tested whether ''~~yddB~~'' and other ICE''Bs1'' genes are required for mating. Cori presented her research with Matt at the 2009 Boston Bacterial Meeting. Cori is now a graduate student at Arizona State University studying microbiology. <br>	+	For CHEM L333, Cori helped clone the ''conB'' gene encoded on the ICE''Bs1'' conjugative element. Cori extended her CHEM L333 project for CHEM L428/L429. She tested whether ''conB'' and other ICE''Bs1'' genes are required for mating. Cori presented her research with Matt at the 2009 Boston Bacterial Meeting. Cori is now a graduate student at Arizona State University studying microbiology. <br>
	<br>		<br>
	'''Erin Cross''' - B.S. Biochemistry, May 2009<br>		'''Erin Cross''' - B.S. Biochemistry, May 2009<br>
-	In CHEM L333, Erin and her class mates attempted to clone various C-terminal truncations of ~~YddE~~ fused to GFP. For CHEM L428/L429, she used fluorescence microscopy to analyze what parts of ~~YddE~~ are required for localization to the cell poles. She found that the C-terminal half of ~~YddE~~ is critical for localization~~. In addition, she found the ConE-GFP localizes at the cell poles, even when the upstream gene ''yddD'' is not expressed ''in cis''~~. She presented this work at the national ACS meeting in March 2009 and at the 2009 Suffolk Science Banquet where her poster won 3rd place. Erin is now working as a laboratory research technologist at MMCRI.<br>	+	In CHEM L333, Erin and her class mates attempted to clone various C-terminal truncations of ConE fused to GFP. For CHEM L428/L429, she used fluorescence microscopy to analyze what parts of ConE are required for localization to the cell poles. She found that the C-terminal half of ConE is critical for localization. She presented this work at the national ACS meeting in March 2009 and at the 2009 Suffolk Science Banquet where her poster won 3rd place. Erin is now working as a laboratory research technologist at MMCRI.<br>
	<br>		<br>
	'''Maria Muccioli (formerly Levicheva)''' - B.S. Biochemistry, Honors Program, May 2009<br>		'''Maria Muccioli (formerly Levicheva)''' - B.S. Biochemistry, Honors Program, May 2009<br>
-	For CHEM L333, Maria ~~began construction of~~ a his-tagged ~~YddE~~. For CHEM L428/L429, she purified and characterized His6-~~YddE~~ to enable future students to perform ATPase assays to determine whether ~~YddE~~ can hydrolyze ATP ''in vitro''. She presented this work at the national ACS meeting in March 2009. Maria is now a graduate student in the molecular and cellular biology Ph.D. program at Ohio University.<br>	+	For CHEM L333, Maria constructed a his-tagged ConE. For CHEM L428/L429, she purified and characterized His6-ConE to enable future students to perform ATPase assays to determine whether ConE can hydrolyze ATP ''in vitro''. She presented this work at the national ACS meeting in March 2009. Maria is now a graduate student in the molecular and cellular biology Ph.D. program at Ohio University.<br>
	<br>		<br>
	'''Tamara Wong''' - B.S. Biochemistry Forensic Science, May 2009<br>		'''Tamara Wong''' - B.S. Biochemistry Forensic Science, May 2009<br>
-	Tamara cloned the His6-~~YddE~~ construct so that we can test whether this protein can support mating. In addition, Tamara purified His6-~~YddE~~ to enable future ATPase assays.<br>	+	Tamara helped cloned the His6-ConE construct so that we can test whether this protein can support mating. In addition, Tamara purified His6-ConE to enable future ATPase assays.<br>
	<br>		<br>
	'''Emma-Kate Loveday''' - B.S. Biochemistry, May 2008<br>		'''Emma-Kate Loveday''' - B.S. Biochemistry, May 2008<br>
-	For her CHEM L428/L429 project, Emma-Kate constructed two variants of ~~YddE~~ and tested their effects on mating. She found that the Walker B (ATP hydrolysis domain) of ~~YddE~~ is essential for mating. She also found that the N-terminus of ~~YddE~~ does not contribute significantly to mating. She presented her work at the Boston Bacterial Meeting in June 2008 and the Cold Spring Harbor Molecular Genetics of Bacteria and Phages Meeting in August 2008. Emma is now a graduate student in the microbiology and immunology Ph.D. program at the University of British Columbia supported by an NSF Fellowship.<br>	+	For her CHEM L428/L429 project, Emma-Kate constructed two variants of ConE and tested their effects on mating. She found that the Walker B (ATP hydrolysis domain) of ConE is essential for mating. She also found that the N-terminus of ConE does not contribute significantly to mating. She presented her work at the Boston Bacterial Meeting in June 2008 and the Cold Spring Harbor Molecular Genetics of Bacteria and Phages Meeting in August 2008. Emma is now a graduate student in the microbiology and immunology Ph.D. program at the University of British Columbia supported by an NSF Fellowship.<br>
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