Berk2010-Daniela: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
mNo edit summary |
mNo edit summary |
||
| Line 1: | Line 1: | ||
= | ==[[User:Daniela Mehech|Daniela Mehech]] 18:58, 7 June 2010 (EDT)== | ||
Did the following with Christoph: <br> | Did the following with Christoph: <br> | ||
Eco/Bam digest of parts: Bjh2245 (LifeACT), Bjh1882 (RFP), and BCA1091 (Ptet) <br> | Eco/Bam digest of parts: Bjh2245 (LifeACT), Bjh1882 (RFP), and BCA1091 (Ptet) <br> | ||
| Line 9: | Line 9: | ||
Did zymo gel purification of Bjh1882 (see image below)<br> | Did zymo gel purification of Bjh1882 (see image below)<br> | ||
[[Image:Gel_purify_bjh1882.JPG|300px]]<br> | [[Image:Gel_purify_bjh1882.JPG|300px]]<br> | ||
Revision as of 15:58, 7 June 2010
Daniela Mehech 18:58, 7 June 2010 (EDT)
Did the following with Christoph:
Eco/Bam digest of parts: Bjh2245 (LifeACT), Bjh1882 (RFP), and BCA1091 (Ptet)
Gel purified Bjh1882 and Bjh2245
Small fragment zymo cleanup of BCA1091
Couldn't see fragments. Turns out Bjh2245 is 97bp so we should have done a small zymo clean up instead of running it on a gel
Re-cut Bjh2245 and Bjh1882
Did a small zymo clean up of Bjh2245
Did zymo gel purification of Bjh1882 (see image below)
