Berk2010-Daniela: Difference between revisions
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[[User:Daniela Mehech|Daniela Mehech]]==[[User:Daniela Mehech|Daniela Mehech]] 18:19, 7 June 2010 (EDT)== | |||
==[[User:Daniela Mehech|Daniela Mehech]] | Did the following with Christoph <br> | ||
Eco/Bam digest of parts: Bjh2245 (LifeACT), Bjh1882 (RFP), and BCA1091 (Ptet) <br> | |||
Gel purified Bjh1882 and Bjh2245 <br> | |||
Small fragment zymo cleanup of BCA1091 <br> | |||
Couldn't see fragments. Turns out Bjh2245 is 97bp and Bjh1882 is ~680bps. <br> | |||
Re-cutting Bjh2245 and Bjh1882. <br> | |||
Small fragment digest of Bjh2245. <br> <br> | |||
[[Image:Gel_purify_bjh1882.JPG|300px]]<br> | |||
Gel purified Bjh1882. <br> | |||
Revision as of 15:19, 7 June 2010
Daniela Mehech==Daniela Mehech 18:19, 7 June 2010 (EDT)==
Did the following with Christoph
Eco/Bam digest of parts: Bjh2245 (LifeACT), Bjh1882 (RFP), and BCA1091 (Ptet)
Gel purified Bjh1882 and Bjh2245
Small fragment zymo cleanup of BCA1091
Couldn't see fragments. Turns out Bjh2245 is 97bp and Bjh1882 is ~680bps.
Re-cutting Bjh2245 and Bjh1882.
Small fragment digest of Bjh2245.
Gel purified Bjh1882.
