Berk2010-Christoph: Difference between revisions
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Bjh2245+pMLL6 <br> | Bjh2245+pMLL6 <br> | ||
Bca1091+pMLL5 <br> | Bca1091+pMLL5 <br> | ||
1091 | 1091 = Lefty <br> | ||
1882 | 1882 = Lefty <br> | ||
Ligated isolated Bjh1882 and Bca1091 into above vectors. Transformed into Lefty strains. <br> | Ligated isolated Bjh1882 and Bca1091 into above vectors. Transformed into Lefty strains. <br> | ||
Need to ligate Bjh2245 (Missing pMLL6, need to digest). <br> | Need to ligate Bjh2245 (Missing pMLL6, need to digest). <br> | ||
Revision as of 17:12, 7 June 2010
Christoph Neyer 15:54, 7 June 2010 (EDT)
Eco/Bam digest of parts: Bjh2245 (LifeACT), Bjh1882 (RFP), and BCA1091 (Ptet).
Gel purified Bjh1882 and Bjh2245.
Small fragment zymo cleanup of BCA1091.
Christoph Neyer 16:35, 7 June 2010 (EDT)
Couldn't see fragments. Turns out Bjh2245 is 97bp and Bjh1882 is ~680bps.
Re-cutting Bjh2245 and Bjh1882.
Small fragment digest of Bjh2245.
Gel purified Bjh1882.
Christoph Neyer 19:30, 7 June 2010 (EDT)
Bjh1882 Vectors: AC
Bjh2245 Vectors: KA
Bca1091 Vectors: CK
pMLL4-AC
pMLL5-CK
pMLL6-KA
pMLL7-AK
pMLL8-KC
pMLL9-CA
Bjh1882+pMLL4
Bjh2245+pMLL6
Bca1091+pMLL5
1091 = Lefty
1882 = Lefty
Ligated isolated Bjh1882 and Bca1091 into above vectors. Transformed into Lefty strains.
Need to ligate Bjh2245 (Missing pMLL6, need to digest).
