Berk2010-Christoph: Difference between revisions
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Gel purified Bjh1882. <br> | Gel purified Bjh1882. <br> | ||
==[[User:Christoph Neyer|Christoph Neyer]] 19:30, 7 June 2010 (EDT)== | ==[[User:Christoph Neyer|Christoph Neyer]] 19:30, 7 June 2010 (EDT)== | ||
Bjh1882 Vectors: AC | Bjh1882 Vectors: AC <br> | ||
Bjh2245 Vectors: KA | Bjh2245 Vectors: KA <br> | ||
Bca1091 Vectors: CK | Bca1091 Vectors: CK <br> | ||
Revision as of 16:30, 7 June 2010
Christoph Neyer 15:54, 7 June 2010 (EDT)
Eco/Bam digest of parts: Bjh2245 (LifeACT), Bjh1882 (RFP), and BCA1091 (Ptet).
Gel purified Bjh1882 and Bjh2245.
Small fragment zymo cleanup of BCA1091.
Christoph Neyer 16:35, 7 June 2010 (EDT)
Couldn't see fragments. Turns out Bjh2245 is 97bp and Bjh1882 is ~680bps.
Re-cutting Bjh2245 and Bjh1882.
Small fragment digest of Bjh2245.
Gel purified Bjh1882.
Christoph Neyer 19:30, 7 June 2010 (EDT)
Bjh1882 Vectors: AC
Bjh2245 Vectors: KA
Bca1091 Vectors: CK
