Berglund:Kinasing: Difference between revisions
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==Kinasing the 5' end of | ==Kinasing the 5' end of purchased RNA oligomers == | ||
*1µl 10X kinase buffer | *1µl 10X kinase buffer | ||
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Incubate at 37°C for 1 hr. | Incubate at 37°C for 1 hr. | ||
Clean with BioRad microspin columns | Clean with [[Berglund:Spin_Column|BioRad microspin columns]]. | ||
Run RNA on [[Berglund:Acrylamide|denaturing gel]]. | Run RNA on [[Berglund:Acrylamide|denaturing gel]]. | ||
Revision as of 17:11, 24 September 2008
Kinasing the 5' end of purchased RNA oligomers
- 1µl 10X kinase buffer
- 5µl water
- 1µl Rnase inhibitor
- 1µl 2µM RNA (fc=.2µM or 2pmoles total)
- 1µl T4 kinase enzyme
- 1µl gamma-ATP (Perkin-Elmer(NEN) BLU-502z)
Incubate at 37°C for 1 hr.
Clean with BioRad microspin columns.
Run RNA on denaturing gel.
