Bacterial transformation - Revision history

Christopher C Vanlang: /* Introduction */

Christopher C Vanlang — Fri, 30 Dec 2011 03:45:51 GMT

Introduction

←Older revision		Revision as of 03:45, 30 December 2011
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	Transformation is the process of introducing foreign DNA (e.g plasmids, BAC) into a bacterium. Bacterial cells into which foreign DNA can be transformed are called '''competent'''. Some bacteria are naturally competent (e.g ''B. subtilis''), whereas others such as ''E. coli'' are not naturally competent. Non-competent cells can be made competent and then transformed via one of two main approaches; '''chemical transformation''' and '''electroporation'''.		Transformation is the process of introducing foreign DNA (e.g plasmids, BAC) into a bacterium. Bacterial cells into which foreign DNA can be transformed are called '''competent'''. Some bacteria are naturally competent (e.g ''B. subtilis''), whereas others such as ''E. coli'' are not naturally competent. Non-competent cells can be made competent and then transformed via one of two main approaches; '''chemical transformation''' and '''electroporation'''.

-	There are advantages and disadvantages to both transformation methods. In general, chemical transformation is less prone to error and faster however electroporation produces a higher transformation efficiency (fraction of transformed cells that actually uptake the foreign DNA). See [[~~Molecular~~ Cloning]] for a fuller discussion of both approaches.	+	There are advantages and disadvantages to both transformation methods. In general, chemical transformation is less prone to error and faster however electroporation produces a higher transformation efficiency (fraction of transformed cells that actually uptake the foreign DNA). See [[Cloning]] for a fuller discussion of both approaches.

	==Protocols==		==Protocols==

Torsten Waldminghaus at 16:58, 23 February 2009

Torsten Waldminghaus — Mon, 23 Feb 2009 16:58:45 GMT

←Older revision		Revision as of 16:58, 23 February 2009
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-	~~''Back~~ to ~~[[Protocols]]''~~	+	{{back to protocols}}
	==Introduction==		==Introduction==
	Transformation is the process of introducing foreign DNA (e.g plasmids, BAC) into a bacterium. Bacterial cells into which foreign DNA can be transformed are called '''competent'''. Some bacteria are naturally competent (e.g ''B. subtilis''), whereas others such as ''E. coli'' are not naturally competent. Non-competent cells can be made competent and then transformed via one of two main approaches; '''chemical transformation''' and '''electroporation'''.		Transformation is the process of introducing foreign DNA (e.g plasmids, BAC) into a bacterium. Bacterial cells into which foreign DNA can be transformed are called '''competent'''. Some bacteria are naturally competent (e.g ''B. subtilis''), whereas others such as ''E. coli'' are not naturally competent. Non-competent cells can be made competent and then transformed via one of two main approaches; '''chemical transformation''' and '''electroporation'''.

Tk: /* References */

Tk — Thu, 25 Sep 2008 22:41:30 GMT

References

←Older revision		Revision as of 22:41, 25 September 2008
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	# Cohen-PNAS-1972 pmid=4559594		# Cohen-PNAS-1972 pmid=4559594
	// This appears to be one of the earliest papers on transformation of Escherichia coli. If you find an earlier paper, please include it here.		// This appears to be one of the earliest papers on transformation of Escherichia coli. If you find an earlier paper, please include it here.
-	# Sharma07 Sharma AD, Singh J, Gill PK; Ethanol mediated enhancement of bacterial transformation, Electronic Journal of Biotechnology, 10(1), DOI: 10.2225/vol10-issue1-fulltext-10, [http://www.ejbiotechnology.info/content/vol10/issue1/full/10/index.html here].	+	# Sharma07 Sharma AD, Singh J, Gill PK; Ethanol mediated enhancement of bacterial transformation, Electronic Journal of Biotechnology (2007), 10(1), DOI: 10.2225/vol10-issue1-fulltext-10, [http://www.ejbiotechnology.info/content/vol10/issue1/full/10/index.html here].

	</biblio>		</biblio>

Tk: /* References */

Tk — Thu, 25 Sep 2008 22:40:38 GMT

References

←Older revision		Revision as of 22:40, 25 September 2008
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	# Cohen-PNAS-1972 pmid=4559594		# Cohen-PNAS-1972 pmid=4559594
	// This appears to be one of the earliest papers on transformation of Escherichia coli. If you find an earlier paper, please include it here.		// This appears to be one of the earliest papers on transformation of Escherichia coli. If you find an earlier paper, please include it here.
		+	# Sharma07 Sharma AD, Singh J, Gill PK; Ethanol mediated enhancement of bacterial transformation, Electronic Journal of Biotechnology, 10(1), DOI: 10.2225/vol10-issue1-fulltext-10, [http://www.ejbiotechnology.info/content/vol10/issue1/full/10/index.html here].
		+
	</biblio>		</biblio>

Tk: /* Chemical transformation */

Tk — Thu, 25 Sep 2008 22:37:13 GMT

Chemical transformation

←Older revision		Revision as of 22:37, 25 September 2008
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	Rubidium chloride transformation protocol [http://wheat.pw.usda.gov/~lazo/methods/goldberg/competen.html here]		Rubidium chloride transformation protocol [http://wheat.pw.usda.gov/~lazo/methods/goldberg/competen.html here]
		+
		+
		+	Someone should check the claims of 1e10 chemical competence using 10% ethanol and calcium chloride protocols [http://www.ejbiotechnology.info/content/vol10/issue1/full/10/index.html here].

	===Electroporation===		===Electroporation===

Tk: /* Chemical transformation */

Tk — Fri, 25 Jul 2008 02:13:19 GMT

Chemical transformation

←Older revision		Revision as of 02:13, 25 July 2008
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	Someone should check out the claims of Nishimura90. [[User:Tk\|tk]] 08:58, 25 September 2007 (EDT)		Someone should check out the claims of Nishimura90. [[User:Tk\|tk]] 08:58, 25 September 2007 (EDT)
		+
		+	Rubidium chloride transformation protocol [http://wheat.pw.usda.gov/~lazo/methods/goldberg/competen.html here]

	===Electroporation===		===Electroporation===

Reshma P. Shetty: /* References */

Reshma P. Shetty — Thu, 20 Dec 2007 20:04:49 GMT

References

←Older revision		Revision as of 20:04, 20 December 2007
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	# Bloom05 US Patent 6,855,494 [[Media:pat6855494.pdf]]		# Bloom05 US Patent 6,855,494 [[Media:pat6855494.pdf]]
	# Jessee05 US Patent 6,960,464 [[Media:pat6960464.pdf]]		# Jessee05 US Patent 6,960,464 [[Media:pat6960464.pdf]]
		+	# Cohen-PNAS-1972 pmid=4559594
		+	// This appears to be one of the earliest papers on transformation of Escherichia coli. If you find an earlier paper, please include it here.
	</biblio>		</biblio>

Tk: /* Chemical transformation */

Tk — Tue, 25 Sep 2007 12:58:49 GMT

Chemical transformation

←Older revision		Revision as of 12:58, 25 September 2007
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	[[Chemical transformation buffer comparison]]		[[Chemical transformation buffer comparison]]
		+
		+	Someone should check out the claims of Nishimura90. [[User:Tk\|tk]] 08:58, 25 September 2007 (EDT)

	===Electroporation===		===Electroporation===

Tk: /* References */

Tk — Tue, 25 Sep 2007 12:57:24 GMT

References

←Older revision		Revision as of 12:57, 25 September 2007
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	#MolecularCloning isbn=0-87969-577-3		#MolecularCloning isbn=0-87969-577-3
	# Hanahan91 pmid=1943786		# Hanahan91 pmid=1943786
		+	# Nishimura90 pmid=2235524
	# Hanahan89 US Patent 4,851,348 [[Media:pat4851348.pdf]]		# Hanahan89 US Patent 4,851,348 [[Media:pat4851348.pdf]]
	# Jessee90 US Patent 4,981,797 [[Media:pat4981797.pdf]]		# Jessee90 US Patent 4,981,797 [[Media:pat4981797.pdf]]

Reshma P. Shetty: /* References */

Reshma P. Shetty — Tue, 08 May 2007 20:19:23 GMT

References

←Older revision		Revision as of 20:19, 8 May 2007
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	</biblio>		</biblio>

-		+	[[Category:Protocol]]
-	[[Category:~~Escherichia coli~~]] [[Category:~~Protocol~~]]	+	[[Category:In vivo]]
		+	[[Category:Escherichia coli]]