Bacterial species identification: Difference between revisions
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#Watanabe01 pmid=11240048 | #Watanabe01 pmid=11240048 | ||
#Bottger89 pmid=2482222 | #Bottger89 pmid=2482222 | ||
Revision as of 18:56, 11 April 2006
- Weisburg WG, Barns SM, Pelletier DA, and Lane DJ. 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol. 1991 Jan;173(2):697-703. DOI:10.1128/jb.173.2.697-703.1991 |
- Wilson KH, Blitchington RB, and Greene RC. Amplification of bacterial 16S ribosomal DNA with polymerase chain reaction. J Clin Microbiol. 1990 Sep;28(9):1942-6. DOI:10.1128/jcm.28.9.1942-1946.1990 |
- Rivas R, Velázquez E, Zurdo-Piñeiro JL, Mateos PF, and Martínez Molina E. Identification of microorganisms by PCR amplification and sequencing of a universal amplified ribosomal region present in both prokaryotes and eukaryotes. J Microbiol Methods. 2004 Mar;56(3):413-26. DOI:10.1016/j.mimet.2003.11.007 |
- Baker GC, Smith JJ, and Cowan DA. Review and re-analysis of domain-specific 16S primers. J Microbiol Methods. 2003 Dec;55(3):541-55. DOI:10.1016/j.mimet.2003.08.009 |
- Watanabe K, Kodama Y, and Harayama S. Design and evaluation of PCR primers to amplify bacterial 16S ribosomal DNA fragments used for community fingerprinting. J Microbiol Methods. 2001 Apr;44(3):253-62. DOI:10.1016/s0167-7012(01)00220-2 |
- Böttger EC. Rapid determination of bacterial ribosomal RNA sequences by direct sequencing of enzymatically amplified DNA. FEMS Microbiol Lett. 1989 Nov;53(1-2):171-6. DOI:10.1016/0378-1097(89)90386-8 |
Mycoplasma universal primers
Johansson K-E, Characterization of mycoplasmas by PCR and sequence analysis iwth universal 16S rDNA primers, Chapter 18, Mycoplasma Protocols, 1998.
- U1F 12-27 ttg atc ctg gct cag gay daa cg
- U2F 327-348 cca rac tcc tac ggr agg c
- U3R 512-527 att acc gcg gck gct g
- U4F 792-810 gta gtc cac gcc gta aac g
- U5R 902-924 ctt gtg cgg gyy ccc gtc aa
- U6F 1154-1172 gag gaa ggy grg gay gay g
- U7R 1359-1374 aca agr ccc gag aac g
- U8R 1502-1524 gaa agg agg trw tcc ayc csc ac
Lane, D. J. (1991). 16S/23S rRNA sequencing. Nucleic acid techniques in bacterial systematics. E. Stackebrandt and M. Goodfellow, eds. New York, NY, John Wiley and Sons: 115-175.
- 27f 5'AGAGTTTGATCMTGGCTCAG
- 342r 5'CTGCTGCSYCCCGTAG
- 357f 5'CTCCTACGGGAGGCAGCAG
- 519r 5'GWATTACCGCGGCKGCTG
- 530f 5'GTGCCAGCMGCCGCGG
- 1100r 5'GGGTTGCGCTCGTTG
- 1114f 5'GCAACGAGCGCAACCC
- 1392r 5'ACGGGCGGTGTGTRC
- 1406f 5'TGYACACACCTCCCGT
- 1492r 5'TACGGYTACCTTGTTACGACTT
- 1525r 5'AAGGAGGTGWTCCARCC
