Bacterial species identification
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==Mycoplasma universal primers== | ==Mycoplasma universal primers== | ||
Revision as of 21:52, 11 April 2006
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Mycoplasma universal primers
Johansson K-E, Characterization of mycoplasmas by PCR and sequence analysis iwth universal 16S rDNA primers, Chapter 18, Mycoplasma Protocols, 1998.
- U1F 12-27 ttg atc ctg gct cag gay daa cg
- U2F 327-348 cca rac tcc tac ggr agg c
- U3R 512-527 att acc gcg gck gct g
- U4F 792-810 gta gtc cac gcc gta aac g
- U5R 902-924 ctt gtg cgg gyy ccc gtc aa
- U6F 1154-1172 gag gaa ggy grg gay gay g
- U7R 1359-1374 aca agr ccc gag aac g
- U8R 1502-1524 gaa agg agg trw tcc ayc csc ac
Lane, D. J. (1991). 16S/23S rRNA sequencing. Nucleic acid techniques in bacterial systematics. E. Stackebrandt and M. Goodfellow, eds. New York, NY, John Wiley and Sons: 115-175.
- 27f 5'AGAGTTTGATCMTGGCTCAG
- 342r 5'CTGCTGCSYCCCGTAG
- 357f 5'CTCCTACGGGAGGCAGCAG
- 519r 5'GWATTACCGCGGCKGCTG
- 530f 5'GTGCCAGCMGCCGCGG
- 1100r 5'GGGTTGCGCTCGTTG
- 1114f 5'GCAACGAGCGCAACCC
- 1392r 5'ACGGGCGGTGTGTRC
- 1406f 5'TGYACACACCTCCCGT
- 1492r 5'TACGGYTACCTTGTTACGACTT
- 1525r 5'AAGGAGGTGWTCCARCC
