Bacterial species identification

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PMID 1987160
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<cite>
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# pmid=1987160
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PMID 2095137
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# pmid=2095137
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PMID 14967233
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# pmid=14967233
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PMID 14607398
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# pmid=14607398
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PMID 11240048
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# pmid=11240048
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PMID 1987160
+
# pmid=1987160
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PMID 2095137
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# pmid=2095137
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PMID  2482222
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# pmid=2482222
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</cite>
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Mycoplasma universal primers
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==Mycoplasma universal primers==
Johansson K-E, Characterization of mycoplasmas by PCR and sequence analysis iwth universal 16S rDNA primers, Chapter 18, Mycoplasma Protocols, 1998.
Johansson K-E, Characterization of mycoplasmas by PCR and sequence analysis iwth universal 16S rDNA primers, Chapter 18, Mycoplasma Protocols, 1998.
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U1F  12-27  ttg atc ctg gct cag gay daa cg
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* U1F  12-27  ttg atc ctg gct cag gay daa cg
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+
* U2F  327-348  cca rac tcc tac ggr agg c
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U2F  327-348  cca rac tcc tac ggr agg c
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* U3R  512-527  att acc gcg gck gct g
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+
* U4F  792-810  gta gtc cac gcc gta aac g
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U3R  512-527  att acc gcg gck gct g
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* U5R  902-924  ctt gtg cgg gyy ccc gtc aa
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* U6F  1154-1172  gag gaa ggy grg gay gay g
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U4F  792-810  gta gtc cac gcc gta aac g
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* U7R  1359-1374  aca agr ccc gag aac g
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* U8R  1502-1524  gaa agg agg trw tcc ayc csc ac
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U5R  902-924  ctt gtg cgg gyy ccc gtc aa
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-
 
+
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U6F  1154-1172  gag gaa ggy grg gay gay g
+
-
 
+
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U7R  1359-1374  aca agr ccc gag aac g
+
-
 
+
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U8R  1502-1524  gaa agg agg trw tcc ayc csc ac
+
Lane, D. J. (1991). 16S/23S rRNA sequencing. Nucleic acid techniques in bacterial systematics. E. Stackebrandt and M. Goodfellow, eds. New York, NY, John Wiley and Sons: 115-175.
Lane, D. J. (1991). 16S/23S rRNA sequencing. Nucleic acid techniques in bacterial systematics. E. Stackebrandt and M. Goodfellow, eds. New York, NY, John Wiley and Sons: 115-175.
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27f 5'AGAGTTTGATCMTGGCTCAG
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* 27f 5'AGAGTTTGATCMTGGCTCAG
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342r 5'CTGCTGCSYCCCGTAG
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* 342r 5'CTGCTGCSYCCCGTAG
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357f 5'CTCCTACGGGAGGCAGCAG
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* 357f 5'CTCCTACGGGAGGCAGCAG
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519r 5'GWATTACCGCGGCKGCTG
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* 519r 5'GWATTACCGCGGCKGCTG
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530f 5'GTGCCAGCMGCCGCGG
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* 530f 5'GTGCCAGCMGCCGCGG
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1100r 5'GGGTTGCGCTCGTTG
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* 1100r 5'GGGTTGCGCTCGTTG
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1114f 5'GCAACGAGCGCAACCC
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* 1114f 5'GCAACGAGCGCAACCC
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1392r 5'ACGGGCGGTGTGTRC
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* 1392r 5'ACGGGCGGTGTGTRC
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1406f 5'TGYACACACCTCCCGT
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* 1406f 5'TGYACACACCTCCCGT
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1492r 5'TACGGYTACCTTGTTACGACTT
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* 1492r 5'TACGGYTACCTTGTTACGACTT
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1525r 5'AAGGAGGTGWTCCARCC
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* 1525r 5'AAGGAGGTGWTCCARCC

Revision as of 20:50, 11 April 2006

[1, 1, 1, 1, 1, 1, 1, 1, 2, 2, 3, 3, 4, 5, 6, 7]

Mycoplasma universal primers

Johansson K-E, Characterization of mycoplasmas by PCR and sequence analysis iwth universal 16S rDNA primers, Chapter 18, Mycoplasma Protocols, 1998.

  • U1F 12-27 ttg atc ctg gct cag gay daa cg
  • U2F 327-348 cca rac tcc tac ggr agg c
  • U3R 512-527 att acc gcg gck gct g
  • U4F 792-810 gta gtc cac gcc gta aac g
  • U5R 902-924 ctt gtg cgg gyy ccc gtc aa
  • U6F 1154-1172 gag gaa ggy grg gay gay g
  • U7R 1359-1374 aca agr ccc gag aac g
  • U8R 1502-1524 gaa agg agg trw tcc ayc csc ac


Lane, D. J. (1991). 16S/23S rRNA sequencing. Nucleic acid techniques in bacterial systematics. E. Stackebrandt and M. Goodfellow, eds. New York, NY, John Wiley and Sons: 115-175.

  • 27f 5'AGAGTTTGATCMTGGCTCAG
  • 342r 5'CTGCTGCSYCCCGTAG
  • 357f 5'CTCCTACGGGAGGCAGCAG
  • 519r 5'GWATTACCGCGGCKGCTG
  • 530f 5'GTGCCAGCMGCCGCGG
  • 1100r 5'GGGTTGCGCTCGTTG
  • 1114f 5'GCAACGAGCGCAACCC
  • 1392r 5'ACGGGCGGTGTGTRC
  • 1406f 5'TGYACACACCTCCCGT
  • 1492r 5'TACGGYTACCTTGTTACGACTT
  • 1525r 5'AAGGAGGTGWTCCARCC
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