Bacterial species identification
From OpenWetWare
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PMID 11240048 | PMID 11240048 | ||
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| + | PMID 1987160 | ||
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| + | PMID 2095137 | ||
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| + | PMID 2482222 | ||
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U8R 1502-1524 gaa agg agg trw tcc ayc csc ac | U8R 1502-1524 gaa agg agg trw tcc ayc csc ac | ||
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| + | Lane, D. J. (1991). 16S/23S rRNA sequencing. Nucleic acid techniques in bacterial systematics. E. Stackebrandt and M. Goodfellow, eds. New York, NY, John Wiley and Sons: 115-175. | ||
| + | |||
| + | 27f 5'AGAGTTTGATCMTGGCTCAG | ||
| + | 342r 5'CTGCTGCSYCCCGTAG | ||
| + | 357f 5'CTCCTACGGGAGGCAGCAG | ||
| + | 519r 5'GWATTACCGCGGCKGCTG | ||
| + | 530f 5'GTGCCAGCMGCCGCGG | ||
| + | 1100r 5'GGGTTGCGCTCGTTG | ||
| + | 1114f 5'GCAACGAGCGCAACCC | ||
| + | 1392r 5'ACGGGCGGTGTGTRC | ||
| + | 1406f 5'TGYACACACCTCCCGT | ||
| + | 1492r 5'TACGGYTACCTTGTTACGACTT | ||
| + | 1525r 5'AAGGAGGTGWTCCARCC | ||
Revision as of 22:11, 7 April 2006
Mycoplasma universal primers
Johansson K-E, Characterization of mycoplasmas by PCR and sequence analysis iwth universal 16S rDNA primers, Chapter 18, Mycoplasma Protocols, 1998.
U1F 12-27 ttg atc ctg gct cag gay daa cg
U2F 327-348 cca rac tcc tac ggr agg c
U3R 512-527 att acc gcg gck gct g
U4F 792-810 gta gtc cac gcc gta aac g
U5R 902-924 ctt gtg cgg gyy ccc gtc aa
U6F 1154-1172 gag gaa ggy grg gay gay g
U7R 1359-1374 aca agr ccc gag aac g
U8R 1502-1524 gaa agg agg trw tcc ayc csc ac
Lane, D. J. (1991). 16S/23S rRNA sequencing. Nucleic acid techniques in bacterial systematics. E. Stackebrandt and M. Goodfellow, eds. New York, NY, John Wiley and Sons: 115-175.
27f 5'AGAGTTTGATCMTGGCTCAG 342r 5'CTGCTGCSYCCCGTAG 357f 5'CTCCTACGGGAGGCAGCAG 519r 5'GWATTACCGCGGCKGCTG 530f 5'GTGCCAGCMGCCGCGG 1100r 5'GGGTTGCGCTCGTTG 1114f 5'GCAACGAGCGCAACCC 1392r 5'ACGGGCGGTGTGTRC 1406f 5'TGYACACACCTCCCGT 1492r 5'TACGGYTACCTTGTTACGACTT 1525r 5'AAGGAGGTGWTCCARCC
