Bacterial species identification

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Revision as of 11:32, 17 March 2007

See here for 16S rRNA alignment assistance.

References

1. Weisburg WG, Barns SM, Pelletier DA, and Lane DJ. . pmid:1987160. PubMed HubMed [Weisburg91]
2. Wilson KH, Blitchington RB, and Greene RC. . pmid:2095137. PubMed HubMed [Wilson90]
3. Rivas R, Velázquez E, Zurdo-Piñeiro JL, Mateos PF, and Martínez Molina E. . pmid:14967233. PubMed HubMed [Rivas04]
4. Baker GC, Smith JJ, and Cowan DA. . pmid:14607398. PubMed HubMed [Baker03]
5. Watanabe K, Kodama Y, and Harayama S. . pmid:11240048. PubMed HubMed [Watanabe01]
6. Böttger EC. . pmid:2482222. PubMed HubMed [Bottger89]

Mycoplasma universal primers

Johansson K-E, Characterization of mycoplasmas by PCR and sequence analysis iwth universal 16S rDNA primers, Chapter 18, Mycoplasma Protocols, 1998.

• U1F 12-27 ttg atc ctg gct cag gay daa cg
• U2F 327-348 cca rac tcc tac ggr agg c
• U3R 512-527 att acc gcg gck gct g
• U4F 792-810 gta gtc cac gcc gta aac g
• U5R 902-924 ctt gtg cgg gyy ccc gtc aa
• U6F 1154-1172 gag gaa ggy grg gay gay g
• U7R 1359-1374 aca agr ccc gag aac g
• U8R 1502-1524 gaa agg agg trw tcc ayc csc ac

Lane, D. J. (1991). 16S/23S rRNA sequencing. Nucleic acid techniques in bacterial systematics. E. Stackebrandt and M. Goodfellow, eds. New York, NY, John Wiley and Sons: 115-175.

• 27f 5'AGAGTTTGATCMTGGCTCAG
• 342r 5'CTGCTGCSYCCCGTAG
• 357f 5'CTCCTACGGGAGGCAGCAG
• 519r 5'GWATTACCGCGGCKGCTG
• 530f 5'GTGCCAGCMGCCGCGG
• 1100r 5'GGGTTGCGCTCGTTG
• 1114f 5'GCAACGAGCGCAACCC
• 1392r 5'ACGGGCGGTGTGTRC
• 1406f 5'TGYACACACCTCCCGT
• 1492r 5'TACGGYTACCTTGTTACGACTT
• 1525r 5'AAGGAGGTGWTCCARCC