Baby cell column

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The baby cell column is a way of getting synchronized bacterial cells (E. coli) which are at more or less the same point in their cell cycel.

Basic idea

Bacteria are attached to glass beads with there flagella. The protein coding for a specailly sticy flagella is introduced on the chromosome behind an inducible promotor. The glass beads with the cells are than put on a column and medium without inductor is run thru it. The new born cells will than not have a flagella and washed of with the medium. Such cells will be "babies" and at the beginning of the cell cycel.

Material

For the set up of the column one needs the following material

Part Company and cat. numberPrice Notes Link
1x Rotilabo®-Luer-tubing connectors, Luer-fitting: LLM, for 2,4mm tubing, 10 pices Roth CT59.1 9.95 € http://www.carlroth.com
1x Rotilabo®-Luer-tubing connectors, Luer-fitting: LLF, for 2,4mm tubing, 10 pices Roth CT63.1 9.95 €
1x Rotilabo®-Mini hose reducers, straight , 2,4 – 4,8 mm, 10 pices Roth CT46.111.95 €
1x Rotilabo®-stoppers with hole, 23-29- 5mm hole, 10 pices Roth Y852.118,90 €
3x Rotilabo®-stand rods 600mm Roth 2378.18,95 €
2x Rotilabo®-retort stand basesRoth 2369.115.20 €
7x Rotilabo®-cross double boss headRoth 2194.17.95 €
5x Rotilabo®-stand clamps, made of malleable iron, 40mmRoth 1995.19.45 €
1x Rotilabo®-tube clamps, Polysulfone (autoclavable)Roth T764.1 32.80 €
1x Corning® Erlenmeyer flask polycarbonate baffled flasks capacity 2000 mL, polypropylene cap (vented), package with 6 picesSigma CLS431256-6EA300 €http://www.sigmaaldrich.com

References

  1. Bates D, Epstein J, Boye E, Fahrner K, Berg H, and Kleckner N. . pmid:15978072. PubMed HubMed [Bates2005MolMicro]
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