BYU iGEM/Notebook/31 January 2011: Difference between revisions
(New page: =January 31 2011= ==Normal ROS levels in <i>E. coli</i>== Hydrogen peroxide: "The activity of alkylhydroperoxide reductase (Ahp) is so high that, although H2O2 is formed endogenously in...) |
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"The activity of alkylhydroperoxide reductase (Ahp) is so high that, although H2O2 is formed endogenously inside aerobic E. coli at a rate of about 15 μM/s, the steady-state concentration does not exceed 20 nM." | "The activity of alkylhydroperoxide reductase (Ahp) is so high that, although H2O2 is formed endogenously inside aerobic E. coli at a rate of about 15 μM/s, the steady-state concentration does not exceed 20 nM." | ||
"When these higher doses of H2O2 saturate Ahp, the H2O2 concentration rises beyond the 0.1 μM threshold for OxyR activation (48, 58). Catalase is strongly induced, and it becomes the primary scavenging enzyme. The katG-encoded catalase of E. coli exhibits a high Km, so it is not saturated by even millimolar doses of H2O2 (60). Importantly, because catalase dismutates H2O2, its turnover rate is not restricted by the availability of reducing equivalents." | |||
Superoxide: | Superoxide: | ||
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"Exponentially growing E. coli contains about 20 μM dimeric cytoplasmic SOD. Given that the rate of O2− formation is approximately 5 μM/s in these cells, the outcome is that the steady-state level of O2− is restricted to approximately 0.1 nM (53)." | "Exponentially growing E. coli contains about 20 μM dimeric cytoplasmic SOD. Given that the rate of O2− formation is approximately 5 μM/s in these cells, the outcome is that the steady-state level of O2− is restricted to approximately 0.1 nM (53)." | ||
Source: [http://www.annualreviews.org/doi/full/10.1146/annurev.biochem.77.061606.161055 | Source: [http://www.annualreviews.org/doi/full/10.1146/annurev.biochem.77.061606.161055 Cellular Defenses against Superoxide and Hydrogen Peroxide] | ||
==Diagram of the final mechanism== | |||
[[Image:IGEM Mechanism.jpg|500px|center|Click on image to read description]] | |||
==Links to Gene Sequences== | |||
[http://www.ncbi.nlm.nih.gov/nuccore/NC_003210?report=genbank&from=2619415&to=2620491&strand=true prfa Gene in listeria (should contain thermoswitch)] | |||
[http://sfx.lib.byu.edu/sfxlcl3?url_ver=Z39.88-2004;url_ctx_fmt=info:ofi/fmt:kev:mtx:ctx;rft_val_fmt=info:ofi/fmt:kev:mtx:journal;rft.atitle=An%20RNA%20thermosensor%20controls%20expression%20of%20virulence%20genes%20in%20Listeria%20monocytogenes;rft.auinit=J;rft.aulast=Johansson;rft.date=2002;rft.epage=561;rft.genre=article;rft.issn=0092-8674;rft.issue=5;rft.spage=551;rft.stitle=CELL;rft.title=CELL;rft.volume=110;rfr_id=info:sid/www.isinet.com:WoK:WOS;rft.au=Mandin,%20P;rft.au=Renzoni,%20A;rft.au=Chiaruttini,%20C;rft.au=Springer,%20M;rft.au=Cossart,%20P An RNA Thermosensor Controls Expression of Virulence Genes in Listeria monocytogenes] | |||
[http://biocyc.org/ECOLI/NEW-IMAGE?type=GENE&object=EG10681 OxyR Information] | |||
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[[../28_January_2011|Previous]] [[../2_February_2011|Next]] | |||
Latest revision as of 15:02, 2 February 2011
|
January 31 2011
Normal ROS levels in E. coli
Hydrogen peroxide:
"The activity of alkylhydroperoxide reductase (Ahp) is so high that, although H2O2 is formed endogenously inside aerobic E. coli at a rate of about 15 μM/s, the steady-state concentration does not exceed 20 nM."
"When these higher doses of H2O2 saturate Ahp, the H2O2 concentration rises beyond the 0.1 μM threshold for OxyR activation (48, 58). Catalase is strongly induced, and it becomes the primary scavenging enzyme. The katG-encoded catalase of E. coli exhibits a high Km, so it is not saturated by even millimolar doses of H2O2 (60). Importantly, because catalase dismutates H2O2, its turnover rate is not restricted by the availability of reducing equivalents."
Superoxide:
"Exponentially growing E. coli contains about 20 μM dimeric cytoplasmic SOD. Given that the rate of O2− formation is approximately 5 μM/s in these cells, the outcome is that the steady-state level of O2− is restricted to approximately 0.1 nM (53)."
Source: Cellular Defenses against Superoxide and Hydrogen Peroxide
Diagram of the final mechanism
Links to Gene Sequences
prfa Gene in listeria (should contain thermoswitch)
An RNA Thermosensor Controls Expression of Virulence Genes in Listeria monocytogenes
[[../28_January_2011|Previous]] [[../2_February_2011|Next]]
