BYU iGEM/Notebook/28 January 2011: Difference between revisions
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Which finds the place that needs flipping and turns it on. | Which finds the place that needs flipping and turns it on. | ||
[[Image:http://openwetware.org/images/a/ae/650px-Flip_Recombinase.JPG]] | [[Image:http://openwetware.org/images/a/ae/650px-Flip_Recombinase.JPG SuperFlp Mechanism]] | ||
Revision as of 13:14, 31 January 2011
Circuit Design
FLp Recombinase Mechanism DNA section that is transcribed normally in the presence of ROS, but there is nothing important downstream. When heated, the promoter inverts (5' to 3' as well as switching strands), leading to transcription in the opposite direction, which contains our desired marker.
IRES Mechanism The promoter on the strand is ROS activated, but the mRNA lacks a Shine Dalgarno sequence. The SD sequence will be inserted by a heat-activated internal ribosomal entry site (IRES) in the proper position to result in translation of the marker protein.
Riboswitch Mechanism The presence of ROS leads to translation of protein A. Protein A is required for translation of our mRNA. The mRNA starts with a heat-activated riboswitch flanked by LoxP sites and followed by the Cre protein. So, when activated by heat, the heat-sensitive riboswitch will be eliminated, allowing constituent translation. The Cre sequence is followed by a the sequence which codes for protein A.
"SuperFlp Mechanism" ROS trans factor followed by Riboswitch followed by Flp recombinace (inversion system) Which finds the place that needs flipping and turns it on.
File:Http://openwetware.org/images/a/ae/650px-Flip Recombinase.JPG SuperFlp Mechanism
