BYU iGEM/Notebook/16 February 2011
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(Difference between revisions)
| Line 2: | Line 2: | ||
#Check PCR | #Check PCR | ||
| - | *if good, restriction enzyme digest | + | #*if good, restriction enzyme digest |
| - | *if not good, PCR again | + | #*if not good, PCR again |
#Plasmid: | #Plasmid: | ||
*extract from E. coli | *extract from E. coli | ||
Revision as of 19:37, 16 February 2011
OxySoxy Team To Do:
- Check PCR
- if good, restriction enzyme digest
- if not good, PCR again
- Plasmid:
- extract from E. coli
- PCR amplification
ΔTS Team To Do:
- PCR amplification of primers
- Plasmid extraction
- Insertion
