BYU iGEM/Notebook/16 February 2011

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==OxySoxy Team To Do:==
==OxySoxy Team To Do:==
-
Check PCR
+
#Check PCR
*if good, restriction enzyme digest
*if good, restriction enzyme digest
*if not good, PCR again
*if not good, PCR again
-
Plasmid:
+
##Plasmid:
*extract from E. coli
*extract from E. coli
*PCR amplification
*PCR amplification

Revision as of 18:36, 16 February 2011

OxySoxy Team To Do:

  1. Check PCR
  • if good, restriction enzyme digest
  • if not good, PCR again
    1. Plasmid:
  • extract from E. coli
  • PCR amplification

ΔTS Team To Do:

  1. PCR amplification of primers
  2. Plasmid extraction
  3. Insertion
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