BYU iGEM/Notebook/16 February 2011: Difference between revisions
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(New page: ==OxySoxy Team To Do:== Check PCR if good, restriction enzyme digest if not good, PCR again Plasmid: *extract from E. coli *PCR amplification ==ΔTS Team To Do:== PCR amplification of ...) |
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Check PCR | Check PCR | ||
if good, restriction enzyme digest | *if good, restriction enzyme digest | ||
if not good, PCR again | *if not good, PCR again | ||
Plasmid: | Plasmid: | ||
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==ΔTS Team To Do:== | ==ΔTS Team To Do:== | ||
PCR amplification of primers | #PCR amplification of primers | ||
Plasmid extraction | #Plasmid extraction | ||
Insertion | #Insertion | ||
Revision as of 16:36, 16 February 2011
OxySoxy Team To Do:
Check PCR
- if good, restriction enzyme digest
- if not good, PCR again
Plasmid:
- extract from E. coli
- PCR amplification
ΔTS Team To Do:
- PCR amplification of primers
- Plasmid extraction
- Insertion
