BYU iGEM/Notebook/16 February 2011

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(New page: ==OxySoxy Team To Do:== Check PCR if good, restriction enzyme digest if not good, PCR again Plasmid: *extract from E. coli *PCR amplification ==ΔTS Team To Do:== PCR amplification of ...)
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Check PCR
Check PCR
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if good, restriction enzyme digest
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*if good, restriction enzyme digest
-
if not good, PCR again
+
*if not good, PCR again
Plasmid:
Plasmid:
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==ΔTS Team To Do:==
==ΔTS Team To Do:==
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PCR amplification of primers
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#PCR amplification of primers
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Plasmid extraction
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#Plasmid extraction
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Insertion
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#Insertion

Revision as of 18:36, 16 February 2011

OxySoxy Team To Do:

Check PCR

  • if good, restriction enzyme digest
  • if not good, PCR again

Plasmid:

  • extract from E. coli
  • PCR amplification

ΔTS Team To Do:

  1. PCR amplification of primers
  2. Plasmid extraction
  3. Insertion
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