BYU iGEM/Notebook/16 February 2011: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
(New page: ==OxySoxy Team To Do:== Check PCR if good, restriction enzyme digest if not good, PCR again Plasmid: *extract from E. coli *PCR amplification ==ΔTS Team To Do:== PCR amplification of ...) |
No edit summary |
||
| (4 intermediate revisions by the same user not shown) | |||
| Line 1: | Line 1: | ||
==OxySoxy Team To Do:== | ==OxySoxy Team To Do:== | ||
Check PCR | #Check PCR | ||
if good, restriction enzyme digest | #*if good, restriction enzyme digest | ||
if not good, PCR again | #*if not good, PCR again | ||
#Plasmid: | |||
Plasmid: | #*extract from E. coli | ||
*extract from E. coli | #*PCR amplification | ||
*PCR amplification | |||
==ΔTS Team To Do:== | ==ΔTS Team To Do:== | ||
PCR amplification of primers | #PCR amplification of primers | ||
Plasmid extraction | #Plasmid extraction | ||
Insertion | #Insertion | ||
Latest revision as of 16:37, 16 February 2011
OxySoxy Team To Do:
- Check PCR
- if good, restriction enzyme digest
- if not good, PCR again
- Plasmid:
- extract from E. coli
- PCR amplification
ΔTS Team To Do:
- PCR amplification of primers
- Plasmid extraction
- Insertion
