BUGSS:Build-a-BUG:2-5

Home        Contact        Internal        Lab Members        Projects        Protocols        Build-a-BUG        Build-a-Gene 2016        Build-a-Gene 2015        Build-a-Gene 2014        Build-a-Gene 2013        Chalk Talks       

Build-a-BUG: Series 2, Session 5

Saturday, April 6, 2013
Noon to 4:00 PM

This is the fifth and final Build-A-BUG workshop in a series of five on yeast. You will learn more about molecular cloning and continue to hone your basic lab techniques, while building a mating type detector. After successful ligations and E. coli transformations in Session 4, some students have verified their constructs with colony PCR; they then grew up and isolated the pDNA. We will now conclude this Build-a-BUG series by transforming S. cerevisae (yeast) cells with our mating type detector pDNA constructs.

Background reading

• Review Session 1, Session 2, Session 3, and Session 4
• Transformation of Saccharomyces cerevisiae and other fungi
• Genetic Transformation of Yeast

Protocols

Materials