BUGSS:Build-a-BUG:2-5

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m (New page: {{BUGSS}} <div style="padding: 10px; width: 780px; border: 5px solid #000000;"> == Build-a-BUG: Series 2, Session 5 == ''Saturday, March 23, 2013''<br> ''Noon to 4:00 PM''<br> This is t...)
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''Noon to 4:00 PM''<br>
''Noon to 4:00 PM''<br>
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This is the fifth Build-A-BUG workshop in a series of five on yeast.  You will learn more about molecular cloning and continue to hone your basic lab techniques, while building a mating type detector.   We will transform ''S. cerevisae'' (yeast) cells with our mating type detector pDNA constructs.<br>
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This is the fifth and final Build-A-BUG workshop in a series of five on yeast.  You will learn more about molecular cloning and continue to hone your basic lab techniques, while building a mating type detector. After successful ligations and ''E. coli'' transformations in Session 4, some students have verified their constructs with colony PCR; they then grew up and isolated the pDNA.  We will now conclude this Build-a-BUG series by transforming ''S. cerevisae'' (yeast) cells with our mating type detector pDNA constructs.<br>
=== Background reading ===
=== Background reading ===

Revision as of 23:15, 1 April 2013

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Contents

Build-a-BUG: Series 2, Session 5

Saturday, March 23, 2013
Noon to 4:00 PM

This is the fifth and final Build-A-BUG workshop in a series of five on yeast. You will learn more about molecular cloning and continue to hone your basic lab techniques, while building a mating type detector. After successful ligations and E. coli transformations in Session 4, some students have verified their constructs with colony PCR; they then grew up and isolated the pDNA. We will now conclude this Build-a-BUG series by transforming S. cerevisae (yeast) cells with our mating type detector pDNA constructs.

Background reading

Protocols

Materials

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