BUGSS:Build-a-BUG:2-5: Difference between revisions
m (New page: {{BUGSS}} <div style="padding: 10px; width: 780px; border: 5px solid #000000;"> == Build-a-BUG: Series 2, Session 5 == ''Saturday, March 23, 2013''<br> ''Noon to 4:00 PM''<br> This is t...) |
mNo edit summary |
||
| Line 7: | Line 7: | ||
''Noon to 4:00 PM''<br> | ''Noon to 4:00 PM''<br> | ||
This is the fifth Build-A-BUG workshop in a series of five on yeast. You will learn more about molecular cloning and continue to hone your basic lab techniques, while building a mating type detector. | This is the fifth and final Build-A-BUG workshop in a series of five on yeast. You will learn more about molecular cloning and continue to hone your basic lab techniques, while building a mating type detector. After successful ligations and ''E. coli'' transformations in Session 4, some students have verified their constructs with colony PCR; they then grew up and isolated the pDNA. We will now conclude this Build-a-BUG series by transforming ''S. cerevisae'' (yeast) cells with our mating type detector pDNA constructs.<br> | ||
=== Background reading === | === Background reading === | ||
Revision as of 20:15, 1 April 2013
Home Contact Internal Lab Members Projects Protocols Build-a-BUG Build-a-Gene 2016 Build-a-Gene 2015 Build-a-Gene 2014 Build-a-Gene 2013 Chalk Talks
Build-a-BUG: Series 2, Session 5
Saturday, March 23, 2013
Noon to 4:00 PM
This is the fifth and final Build-A-BUG workshop in a series of five on yeast. You will learn more about molecular cloning and continue to hone your basic lab techniques, while building a mating type detector. After successful ligations and E. coli transformations in Session 4, some students have verified their constructs with colony PCR; they then grew up and isolated the pDNA. We will now conclude this Build-a-BUG series by transforming S. cerevisae (yeast) cells with our mating type detector pDNA constructs.
Background reading
- Review Session 1, Session 2, Session 3, and Session 4
- Transformation of Saccharomyces cerevisiae and other fungi
- Genetic Transformation of Yeast
