BUGSS:Build-a-BUG:2-3

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=== Background reading ===
=== Background reading ===
* Review [[BUGSS:Build-a-BUG:2-1 | Session 1]] and [[BUGSS:Build-a-BUG:2-2 | Session 2]]
* Review [[BUGSS:Build-a-BUG:2-1 | Session 1]] and [[BUGSS:Build-a-BUG:2-2 | Session 2]]
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* [http://www.ncbi.nlm.nih.gov/pubmed/4377758 DNA ligase: structure, mechanism, and function] (Requires subscription; I will look for an open access alternative)
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* [http://www.ncbi.nlm.nih.gov/projects/genome/probe/doc/TechPCR.shtml Polymerase Chain Reaction (PCR)]
=== Protocols ===
=== Protocols ===
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* [https://www.neb.com/protocols/1/01/01/dna-ligation-with-t4-dna-ligase-m0202 DNA ligation with T4 DNA Ligase]
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* Polymerase Chain Reaction (PCR)
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* [http://carroll.molbio.wisc.edu/methods/Bacterial/CompetentCells.html Making competent ''E. coli''] (BUGSS has done this for you)
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** PCR Master Mix
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***
=== Materials ===
=== Materials ===

Revision as of 14:51, 18 March 2013

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Contents

Build-a-BUG: Series 2, Session 3

Saturday, March 9, 2013
Noon to 4:00 PM

This is the third Build-A-BUG workshop in a series of five on yeast. You will learn more about molecular cloning and continue to hone your basic lab techniques, while continuing either your own or somebody else's mating type detector project.

Background reading

Protocols

  • Polymerase Chain Reaction (PCR)
    • PCR Master Mix

Materials

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