BUGSS:Build-a-BUG:2-3
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* [http://carroll.molbio.wisc.edu/methods/Bacterial/CompetentCells.html Making competent ''E. coli''] (BUGSS has done this for you) | * [http://carroll.molbio.wisc.edu/methods/Bacterial/CompetentCells.html Making competent ''E. coli''] (BUGSS has done this for you) | ||
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* [https://www.neb.com/products/m0202-t4-dna-ligase T4 DNA Ligase] | * [https://www.neb.com/products/m0202-t4-dna-ligase T4 DNA Ligase] | ||
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Revision as of 01:51, 4 March 2013
Contents |
Build-a-BUG: Series 2, Session 3
Saturday, March 9, 2013
Noon to 4:00 PM
This is the third Build-A-BUG workshop in a series of five on yeast. You will learn more about molecular cloning and continue to hone your basic lab techniques, while continuing either your own or somebody else's mating type detector project. This lab will involve ligations (linking DNA) and transformations of competent E. coli cells.
Background reading
- Review Session 1 and Session 2
- DNA ligase: structure, mechanism, and function (Requires subscription; I will look for an open access alternative)
Protocols
- DNA ligation with T4 DNA Ligase
- Making competent E. coli (BUGSS has done this for you)
