BUGSS:Build-a-BUG:2-3
From OpenWetWare
(Difference between revisions)
m |
m |
||
| Line 11: | Line 11: | ||
=== Background reading === | === Background reading === | ||
* Review [[BUGSS:Build-a-BUG:2-1 | Session 1]] and [[BUGSS:Build-a-BUG:2-2 | Session 2]] | * Review [[BUGSS:Build-a-BUG:2-1 | Session 1]] and [[BUGSS:Build-a-BUG:2-2 | Session 2]] | ||
| + | * [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC224649/pdf/pnas00115-0175.pdf Enzymatic breakage and joining of deoxyribonucleic acid, I. Repair of single-strand breaks in DNA by an enzyme system from Escherichia coli infected with T4 bacteriophage.] | ||
=== Protocols === | === Protocols === | ||
Revision as of 01:22, 4 March 2013
Contents |
Build-a-BUG: Series 2, Session 3
Saturday, March 9, 2013
Noon to 4:00 PM
This is the third Build-A-BUG workshop in a series of five on yeast. You will learn more about molecular cloning and continue to hone your basic lab techniques, while continuing either your own or somebody else's mating type detector project. This lab will involve ligations (linking DNA) and transformations of competent E. coli cells.
Background reading
- Review Session 1 and Session 2
- Enzymatic breakage and joining of deoxyribonucleic acid, I. Repair of single-strand breaks in DNA by an enzyme system from Escherichia coli infected with T4 bacteriophage.
