BUGSS:Build-a-BUG:2-3: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
mNo edit summary |
mNo edit summary |
||
| Line 17: | Line 17: | ||
* [http://carroll.molbio.wisc.edu/methods/Bacterial/CompetentCells.html Making competent ''E. coli''] (BUGSS has done this for you) | * [http://carroll.molbio.wisc.edu/methods/Bacterial/CompetentCells.html Making competent ''E. coli''] (BUGSS has done this for you) | ||
=== | === Materials === | ||
* [https://www.neb.com/products/m0202-t4-dna-ligase T4 DNA Ligase] | * [https://www.neb.com/products/m0202-t4-dna-ligase T4 DNA Ligase] | ||
</div> | </div> | ||
Revision as of 22:51, 3 March 2013
Home Contact Internal Lab Members Projects Protocols Build-a-BUG Build-a-Gene 2016 Build-a-Gene 2015 Build-a-Gene 2014 Build-a-Gene 2013 Chalk Talks
Build-a-BUG: Series 2, Session 3
Saturday, March 9, 2013
Noon to 4:00 PM
This is the third Build-A-BUG workshop in a series of five on yeast. You will learn more about molecular cloning and continue to hone your basic lab techniques, while continuing either your own or somebody else's mating type detector project. This lab will involve ligations (linking DNA) and transformations of competent E. coli cells.
Background reading
- Review Session 1 and Session 2
- DNA ligase: structure, mechanism, and function (Requires subscription; I will look for an open access alternative)
Protocols
- DNA ligation with T4 DNA Ligase
- Making competent E. coli (BUGSS has done this for you)
