BUGSS:Build-a-BUG:2-2

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Build-a-BUG: Series 2, Session 2

Saturday, February 23, 2013
Noon to 4:00 PM

This is the second Build-A-BUG workshop in a series of five on yeast. You will continue to hone your basic lab techniques, including pipetting and centrifugation, while continuing either your own or somebody else's mating type detector project. This lab will involve restriction digests (cutting DNA), agarose gel electrophoresis (visualizing DNA), gel extractions (recovering DNA fragments), and possibly ligations (linking DNA).

Background reading

• Review Session 1

Protocols

• Optimizing Restriction Endonuclease Reactions
• DNA ligation with T4 DNA Ligase
• QIAquick Gel Extraction Protocol and Handbook

Restriction endonucleases & DNA ligase

• EcoRI
• XbaI
• SpeI
• PstI
• T4 DNA Ligase