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=== Background reading ===
=== Background reading ===
* Review [[BUGSS:Build-a-BUG:2-1 | Session 1]]
* Review [[BUGSS:Build-a-BUG:2-1 | Session 1]]
* [http://nar.oxfordjournals.org/content/29/18/3705.full.pdf Structure and function of type II restriction endonucleases]
=== Protocols ===
=== Protocols ===

Revision as of 17:37, 18 February 2013


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Build-a-BUG: Series 2, Session 2

Saturday, February 23, 2013
Noon to 4:00 PM

This is the second Build-A-BUG workshop in a series of five on yeast. You will continue to hone your basic lab techniques, including pipetting and centrifugation, while continuing either your own or somebody else's mating type detector project. This lab will involve restriction digests (cutting DNA), agarose gel electrophoresis (visualizing DNA), gel extractions (recovering DNA fragments), and possibly ligations (linking DNA).

Background reading


Restriction endonucleases & DNA ligase

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