BUGSS:Build-a-BUG:2-2

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=== Protocols ===
=== Protocols ===
* [https://www.neb.com/protocols/2012/12/07/optimizing-restriction-endonuclease-reactions Optimizing Restriction Endonuclease Reactions]
* [https://www.neb.com/protocols/2012/12/07/optimizing-restriction-endonuclease-reactions Optimizing Restriction Endonuclease Reactions]
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* [https://www.neb.com/protocols/1/01/01/dna-ligation-with-t4-dna-ligase-m0202 DNA ligation with T4 DNA Ligase]
* [http://openwetware.org/images/f/fe/QIAquick-Gel-Extraction-Kit-EN.pdf QIAquick Gel Extraction Protocol] and [http://openwetware.org/images/7/7f/EN-QIAquick-Spin-Handbook.pdf Handbook]
* [http://openwetware.org/images/f/fe/QIAquick-Gel-Extraction-Kit-EN.pdf QIAquick Gel Extraction Protocol] and [http://openwetware.org/images/7/7f/EN-QIAquick-Spin-Handbook.pdf Handbook]

Revision as of 17:26, 18 February 2013

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Contents

Build-a-BUG: Series 2, Session 2

Saturday, February 23, 2013
Noon to 4:00 PM

This is the second Build-A-BUG workshop in a series of five on yeast. You will continue to hone your basic lab techniques, including pipetting and centrifugation, while continuing either your own or somebody else's mating type detector project. This lab will involve restriction digests (cutting DNA), agarose gel electrophoresis (visualizing DNA), gel extractions (recovering DNA fragments), and possibly ligations (linking DNA).

Background reading

Protocols

Restriction endonucleases & DNA ligase

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