BUGSS:Build-a-BUG:2-2

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m (New page: {{BUGSS}} <div style="padding: 10px; width: 780px; border: 5px solid #000000;"> == Build-a-BUG: Series 2, Session 2 == ''Saturday, February 23, 2013''<br> ''Noon to 4:00 PM''<br> This i...)
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''Noon to 4:00 PM''<br>
''Noon to 4:00 PM''<br>
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This is the second Build-A-BUG workshop in a series of five on yeast.  You will continue to hone your basic lab techniques, including pipetting and centrifugation, while continuing either your own or somebody else's mating type detector project.  This lab will involve restriction digests (cutting DNA), agarose gel electrophoresis (visualizing DNA), and gel extractions (recovering DNA fragments).<br>
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This is the second Build-A-BUG workshop in a series of five on yeast.  You will continue to hone your basic lab techniques, including pipetting and centrifugation, while continuing either your own or somebody else's mating type detector project.  This lab will involve restriction digests (cutting DNA), agarose gel electrophoresis (visualizing DNA), gel extractions (recovering DNA fragments), and possibly ligations (linking DNA).<br>
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=== Background reading ===
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* Review [[BUGSS:Build-a-BUG:2-1 | Session 1]]
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=== Protocols ===
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*
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=== Restriction enzymes & DNA ligase ===
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* [https://www.neb.com/products/r0101-ecori EcoRI]
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* [https://www.neb.com/products/r0145-xbai XbaI]
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* [https://www.neb.com/products/r0133-spei SpeI]
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* [https://www.neb.com/products/r0140-psti PstI]
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* [https://www.neb.com/products/m0202-t4-dna-ligase T4 DNA Ligase]
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Revision as of 18:14, 18 February 2013

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Contents

Build-a-BUG: Series 2, Session 2

Saturday, February 23, 2013
Noon to 4:00 PM

This is the second Build-A-BUG workshop in a series of five on yeast. You will continue to hone your basic lab techniques, including pipetting and centrifugation, while continuing either your own or somebody else's mating type detector project. This lab will involve restriction digests (cutting DNA), agarose gel electrophoresis (visualizing DNA), gel extractions (recovering DNA fragments), and possibly ligations (linking DNA).

Background reading

Protocols

Restriction enzymes & DNA ligase

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