BUGSS:Build-a-BUG:2-2: Difference between revisions
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m (New page: {{BUGSS}} <div style="padding: 10px; width: 780px; border: 5px solid #000000;"> == Build-a-BUG: Series 2, Session 2 == ''Saturday, February 23, 2013''<br> ''Noon to 4:00 PM''<br> This i...) |
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''Noon to 4:00 PM''<br> | ''Noon to 4:00 PM''<br> | ||
This is the second Build-A-BUG workshop in a series of five on yeast. You will continue to hone your basic lab techniques, including pipetting and centrifugation, while continuing either your own or somebody else's mating type detector project. This lab will involve restriction digests (cutting DNA), agarose gel electrophoresis (visualizing DNA), | This is the second Build-A-BUG workshop in a series of five on yeast. You will continue to hone your basic lab techniques, including pipetting and centrifugation, while continuing either your own or somebody else's mating type detector project. This lab will involve restriction digests (cutting DNA), agarose gel electrophoresis (visualizing DNA), gel extractions (recovering DNA fragments), and possibly ligations (linking DNA).<br> | ||
=== Background reading === | |||
* Review [[BUGSS:Build-a-BUG:2-1 | Session 1]] | |||
=== Protocols === | |||
* | |||
=== Restriction enzymes & DNA ligase === | |||
* [https://www.neb.com/products/r0101-ecori EcoRI] | |||
* [https://www.neb.com/products/r0145-xbai XbaI] | |||
* [https://www.neb.com/products/r0133-spei SpeI] | |||
* [https://www.neb.com/products/r0140-psti PstI] | |||
* [https://www.neb.com/products/m0202-t4-dna-ligase T4 DNA Ligase] | |||
</div> | </div> | ||
Revision as of 15:14, 18 February 2013
Home Contact Internal Lab Members Projects Protocols Build-a-BUG Build-a-Gene 2016 Build-a-Gene 2015 Build-a-Gene 2014 Build-a-Gene 2013 Chalk Talks
Build-a-BUG: Series 2, Session 2
Saturday, February 23, 2013
Noon to 4:00 PM
This is the second Build-A-BUG workshop in a series of five on yeast. You will continue to hone your basic lab techniques, including pipetting and centrifugation, while continuing either your own or somebody else's mating type detector project. This lab will involve restriction digests (cutting DNA), agarose gel electrophoresis (visualizing DNA), gel extractions (recovering DNA fragments), and possibly ligations (linking DNA).
Background reading
- Review Session 1
