BUGSS:Build-a-BUG:2-2: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
mNo edit summary |
mNo edit summary |
||
| Line 16: | Line 16: | ||
* [https://www.neb.com/protocols/2012/12/07/optimizing-restriction-endonuclease-reactions Optimizing Restriction Endonuclease Reactions] | * [https://www.neb.com/protocols/2012/12/07/optimizing-restriction-endonuclease-reactions Optimizing Restriction Endonuclease Reactions] | ||
* [https://www.neb.com/protocols/1/01/01/dna-ligation-with-t4-dna-ligase-m0202 DNA ligation with T4 DNA Ligase] | * [https://www.neb.com/protocols/1/01/01/dna-ligation-with-t4-dna-ligase-m0202 DNA ligation with T4 DNA Ligase] | ||
* [[Agarose_gel_electrophoresis]] | * [[Agarose_gel_electrophoresis | Agarose gel electrophoresis]] | ||
* [http://openwetware.org/images/f/fe/QIAquick-Gel-Extraction-Kit-EN.pdf QIAquick Gel Extraction Protocol] and [http://openwetware.org/images/7/7f/EN-QIAquick-Spin-Handbook.pdf Handbook] | * [http://openwetware.org/images/f/fe/QIAquick-Gel-Extraction-Kit-EN.pdf QIAquick Gel Extraction Protocol] and [http://openwetware.org/images/7/7f/EN-QIAquick-Spin-Handbook.pdf Handbook] | ||
Revision as of 15:53, 18 February 2013
Home Contact Internal Lab Members Projects Protocols Build-a-BUG Build-a-Gene 2016 Build-a-Gene 2015 Build-a-Gene 2014 Build-a-Gene 2013 Chalk Talks
Build-a-BUG: Series 2, Session 2
Saturday, February 23, 2013
Noon to 4:00 PM
This is the second Build-A-BUG workshop in a series of five on yeast. You will continue to hone your basic lab techniques, including pipetting and centrifugation, while continuing either your own or somebody else's mating type detector project. This lab will involve restriction digests (cutting DNA), agarose gel electrophoresis (visualizing DNA), gel extractions (recovering DNA fragments), and possibly ligations (linking DNA).
Background reading
Protocols
- Optimizing Restriction Endonuclease Reactions
- DNA ligation with T4 DNA Ligase
- Agarose gel electrophoresis
- QIAquick Gel Extraction Protocol and Handbook
