BUGSS:Build-a-BUG:2-1

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=== Protocols ===
=== Protocols ===
* [http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0023457 One-Step Miniprep]
* [http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0023457 One-Step Miniprep]
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* [http://openwetware.org/images/e/e3/EN-QuickLyse-Miniprep-Handbook.pdf QIAGEN QuickLyse Miniprep]: Read the Introduction (pp. 8-9) and Protocol (pp. 14-15)
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* [http://openwetware.org/images/e/e3/EN-QuickLyse-Miniprep-Handbook.pdf QIAGEN QuickLyse Miniprep]
=== BioBrick parts ===
=== BioBrick parts ===

Revision as of 11:56, 18 February 2013

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Contents

Build-a-BUG: Series 2, Session 1

Saturday, February 9, 2013
Noon to 4:00 PM

This is the first Build-A-BUG workshop in a series of five on yeast. You will be introduced to basic lab techniques, including pipetting and centrifugation, and start your own mating type detector project. This lab will involve crash courses on molecular and cellular biology, the life cycle of budding yeast, and BioBrick-based approaches to synthetic biology, as well as hands-on minipreps (DNA isolation).

Background reading

Protocols

BioBrick parts

Whiteboard

Yeast life cycle and sporulation
Yeast life cycle and sporulation
QuickLyse and One-Step miniprep protocols
QuickLyse and One-Step miniprep protocols
BioBrick parts list
BioBrick parts list
Assembly strategy (pre-cut)
Assembly strategy (pre-cut)
Assembly strategy (post-cut)
Assembly strategy (post-cut)
Personal tools