BUGSS:Build-a-BUG:2-1

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=== BioBrick parts ===
=== BioBrick parts ===
* [http://partsregistry.org/wiki/index.php?title=Part:BBa_K801000 BBa_K801000]: pTUM100 yeast shuttle vector based on pYES2
* [http://partsregistry.org/wiki/index.php?title=Part:BBa_K801000 BBa_K801000]: pTUM100 yeast shuttle vector based on pYES2
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* [http://partsregistry.org/wiki/index.php?title=Part:BBa_K110006 BBa_K110006]: Alpha-Cell Promoter MF(ALPHA)6
+
* [http://partsregistry.org/wiki/index.php?title=Part:BBa_K110006 BBa_K110006]: Alpha-Cell Promoter MF(ALPHA)1
* [http://partsregistry.org/wiki/index.php?title=Part:BBa_K110005 BBa_K110005]: Alpha-Cell Promoter MF(ALPHA)2
* [http://partsregistry.org/wiki/index.php?title=Part:BBa_K110005 BBa_K110005]: Alpha-Cell Promoter MF(ALPHA)2
* [http://partsregistry.org/wiki/index.php?title=Part:BBa_K165057 BBa_K165057]: Kozak + mCherry translational unit
* [http://partsregistry.org/wiki/index.php?title=Part:BBa_K165057 BBa_K165057]: Kozak + mCherry translational unit

Revision as of 23:50, 17 February 2013

Image:BUGSS.png

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Contents

Build-a-BUG: Series 2, Session 1

Saturday, February 9, 2013
Noon to 4:00 PM

This is the first Build-A-BUG workshop in a series of five on yeast. You will be introduced to basic lab techniques, including pipetting and centrifugation, and start your own mating type detector project. This lab will involve crash courses on molecular and cellular biology, the life cycle of budding yeast, and BioBrick-based approaches to synthetic biology, as well as hands-on minipreps (DNA isolation).

Background reading

Protocols

BioBrick parts

Whiteboard

Yeast life cycle and sporulation
Yeast life cycle and sporulation
QuickLyse and One-Step miniprep protocols
QuickLyse and One-Step miniprep protocols
BioBrick parts list
BioBrick parts list
Assembly strategy (pre-cut)
Assembly strategy (pre-cut)
Assembly strategy (post-cut)
Assembly strategy (post-cut)
Personal tools