BUGSS:Build-a-BUG:2-1

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
m
m
Line 30: Line 30:
</div>
</div>
-
=== Whiteboard ===
+
== Whiteboard ==
[[Image:BioBricks.jpg|thumb|left|700px|alt=BioBrick parts list|BioBrick parts list]]
[[Image:BioBricks.jpg|thumb|left|700px|alt=BioBrick parts list|BioBrick parts list]]
[[Image:Pre-cut_assembly.jpg|thumb|left|700px|alt=Assembly strategy (pre-cut)|Assembly strategy (pre-cut)]]
[[Image:Pre-cut_assembly.jpg|thumb|left|700px|alt=Assembly strategy (pre-cut)|Assembly strategy (pre-cut)]]

Revision as of 23:30, 17 February 2013

Image:BUGSS.png

Home        Contact        Internal        Lab Members        Projects        Protocols        Build-a-BUG        Build-a-Gene 2014        Build-a-Gene 2013        Chalk Talks       


Contents

Build-a-BUG: Series 2, Session 1

Saturday, February 9, 2013
Noon to 4:00 PM

This is the first Build-A-BUG workshop in a series of five on yeast. You will be introduced to basic lab techniques, including pipetting and centrifugation, and start your own mating type detector project. This lab will involve crash courses on molecular and cellular biology, the life cycle of budding yeast, and BioBrick-based approaches to synthetic biology, as well as hands-on minipreps (DNA isolation).

Background reading

Protocols

BioBrick parts

Whiteboard

BioBrick parts list
BioBrick parts list
Assembly strategy (pre-cut)
Assembly strategy (pre-cut)
Assembly strategy (post-cut)
Assembly strategy (post-cut)
QuickLyse and One-Step miniprep protocols
QuickLyse and One-Step miniprep protocols
Yeast life cycle and sporulation
Yeast life cycle and sporulation
Personal tools