BUGSS:Build-a-BUG:2-1

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* [http://partsregistry.org/wiki/index.php?title=Part:BBa_K165059 BBa_K165059]: Kozak + CFPx2 translational unit
* [http://partsregistry.org/wiki/index.php?title=Part:BBa_K165059 BBa_K165059]: Kozak + CFPx2 translational unit
* [http://partsregistry.org/wiki/index.php?title=Part:BBa_J63002 BBa_J63002]: ADH1 terminator from ''S. cerevisiae''
* [http://partsregistry.org/wiki/index.php?title=Part:BBa_J63002 BBa_J63002]: ADH1 terminator from ''S. cerevisiae''
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=== Whiteboard ===
 
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[[Image:BioBricks.jpg|thumb|left|550px|alt=BioBrick parts list|BioBrick parts list]]
 
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[[Image:Pre-cut_assembly.jpg|thumb|left|550px|alt=Assembly strategy (pre-cut)|Assembly strategy (pre-cut)]]
 
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[[Image:Post-cut_assembly.jpg|thumb|left|550px|alt=Assembly strategy (post-cut)|Assembly strategy (post-cut)]]
 
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[[Image:Protocols.jpg|thumb|left|550px|alt=QuickLyse and One-Step miniprep protocols|QuickLyse and One-Step miniprep protocols]]
 
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[[Image:Yeast_life_cycle.jpg|thumb|left|550px|alt=Yeast life cycle and sporulation|Yeast life cycle and sporulation]]
 
</div>
</div>
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=== Whiteboard ===
 +
[[Image:BioBricks.jpg|thumb|left|700px|alt=BioBrick parts list|BioBrick parts list]]
 +
[[Image:Pre-cut_assembly.jpg|thumb|left|700px|alt=Assembly strategy (pre-cut)|Assembly strategy (pre-cut)]]
 +
[[Image:Post-cut_assembly.jpg|thumb|left|700px|alt=Assembly strategy (post-cut)|Assembly strategy (post-cut)]]
 +
[[Image:Protocols.jpg|thumb|left|700px|alt=QuickLyse and One-Step miniprep protocols|QuickLyse and One-Step miniprep protocols]]
 +
[[Image:Yeast_life_cycle.jpg|thumb|left|700px|alt=Yeast life cycle and sporulation|Yeast life cycle and sporulation]]

Revision as of 00:29, 18 February 2013

Image:BUGSS.png

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Contents

Build-a-BUG: Series 2, Session 1

Saturday, February 9, 2013
Noon to 4:00 PM

This is the first Build-A-BUG workshop in a series of five on yeast. You will be introduced to basic lab techniques, including pipetting and centrifugation, and start your own mating type detector project. This lab will involve crash courses on molecular and cellular biology, the life cycle of budding yeast, and BioBrick-based approaches to synthetic biology, as well as hands-on minipreps (DNA isolation).

Background reading

Protocols

BioBrick parts

Whiteboard

BioBrick parts list
BioBrick parts list
Assembly strategy (pre-cut)
Assembly strategy (pre-cut)
Assembly strategy (post-cut)
Assembly strategy (post-cut)
QuickLyse and One-Step miniprep protocols
QuickLyse and One-Step miniprep protocols
Yeast life cycle and sporulation
Yeast life cycle and sporulation
Personal tools