BME494s2013 Project Template
Overview & Purpose
Design: Our genetic circuit
OUR GENE SWITCH:
Building: Assembly Scheme
Testing: Modeling and GFP Imaging
For our testing, we will examine the relationship between the level of concentration of the chemical compound toulene and the level of fluorescence. The fluorescence from DH5α cells harboring the constructed biobrick with the xylr protein will be measured at various concentrations of toluene.
For tuning our system, we hope to make it more effective by increasing the efficiency of the ribosome binding site. The RBS controls the accuracy and efficiency with which the translation of mRNA begins. Therefore, we hope to use this important variable to tune our system.
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