BME494s2013 Project Template

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(Building: Assembly Scheme)
(Testing: Modeling and GFP Imaging)
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'''Measurement'''
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'''A LAC SWITCH MODEL'''
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<!--How are you measuring function? Which fluorescence system? Describe procedure-->
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We used a previously published synthetic switch, developed by Ceroni et al., to understand how our system could potentially be modeled and simulated.
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<!-- Continue this paragraph by explaining to a non-specialist what a mathematical model is and what parameter values are, in general. Include your network diagram illustration of the Ceroni et al. model and list all of the parameters you were able to map onto the model -->
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For our testing, we will examine the relationship between the level of concentration of the chemical compound toulene and the level of fluorescence. The fluorescence from DH5α cells harboring the constructed biobrick with the xylr protein will be measured at various concentrations of toluene.
 
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'''AN INTERACTIVE MODEL'''
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'''Expected Observations [4]'''
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We used a model of the natural Lac operon to understand how changing the parameter values changes the behavior of the system.
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<!-- Continue this paragraph by explaining how you interacted with the MatLab model. Include two or more images showing different output curves that were generated when you altered the IPTG concentration -->
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'''COLLECTING IMPERICAL VALUES TO IMPROVE THE MODEL'''
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<!--Make some "mock data" to demonstrate sound experimental design (controls, significance, etc.)-->
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We explored how one technique, imaging via microscopy could be used to determine the production rate of an output protein, in this case GFP in yeast, could be used to determine a "real" value for maximum GFP production rate under our own laboratory conditions.
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[[Image:group1testing01.jpg|400px]] [[Image:group1testing02.jpg|400px]]
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<!-- Describe summarize how you measured GFP intensities, plotted charts in MatLab, make a curve of best fit, and tried to determine the maximum rate of GFP production. You don't have to include your raw values, just the graphs and equations. You may include some small images of the GFP-expressing yeast. If you didn't get a nice peak, explain how your image analysis might be changed to improve your outcome. -->
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'''Tuning Our System'''
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Ideally, the GFP production rate measured by this method could be entered as a value for [which parameter] in the Ceroni et al. model.
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For tuning our system, we hope to make it more effective by increasing the efficiency of the ribosome binding site. The RBS controls the accuracy and efficiency with which the translation of mRNA begins. Therefore, we hope to use this important variable to tune our system.
 
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<!--Explain how you have built in "tunability". Explain expected changes in data. Draw how the graphs would change -->
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'''CONCLUSION'''
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[[Image:group1testing2.jpg|400px]]  [[Image:group1testing03.jpg|400px]]
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Revision as of 18:07, 22 April 2013


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Contents

Overview & Purpose

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Background

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Design: Our genetic circuit

OUR GENE SWITCH:


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Building: Assembly Scheme













Testing: Modeling and GFP Imaging


A LAC SWITCH MODEL
We used a previously published synthetic switch, developed by Ceroni et al., to understand how our system could potentially be modeled and simulated.


AN INTERACTIVE MODEL
We used a model of the natural Lac operon to understand how changing the parameter values changes the behavior of the system.


COLLECTING IMPERICAL VALUES TO IMPROVE THE MODEL
We explored how one technique, imaging via microscopy could be used to determine the production rate of an output protein, in this case GFP in yeast, could be used to determine a "real" value for maximum GFP production rate under our own laboratory conditions.



Ideally, the GFP production rate measured by this method could be entered as a value for [which parameter] in the Ceroni et al. model.



CONCLUSION



Human Practices

Danger of Chemicals in Farmlands
Danger of Chemicals in Farmlands



Our Team

Your Name
Your Name


  • My name is ###, and I am a ### majoring in ###. I am taking BME 494 because ###. An interesting fact about me is that ###.



Your Name
Your Name


  • My name is ###, and I am a ### majoring in ###. I am taking BME 494 because ###. An interesting fact about me is that ###.



Your Name
Your Name


  • My name is ###, and I am a ### majoring in ###. I am taking BME 494 because ###. An interesting fact about me is that ###.



Your Name
Your Name


  • My name is ###, and I am a ### majoring in ###. I am taking BME 494 because ###. An interesting fact about me is that ###.






Works Cited

[1] Full reference.

[2] Full reference.

[3] Full reference.