By modifying the input and output for the Lac switch, it may be possible to produce materials such as plastics. The switch could be triggered by another environmental factor other than [IPTG], and instead of producing GFP, more useful materials like plastic could be an output. Currently, production of plastics is a very energy intensive process, and by using bacteria for the production, we can save energy and limit waste into the environment.
Background
Diagram of Lac operon and how it functions without lactose present (top) and with lactose present (bottom)
Design: Our genetic circuit
OUR GENE SWITCH:
AND gate Gene Switch: Both [IPTG] AND [Low Glucose Levels] conditions must be met for GFP production
Building: Assembly Scheme
Testing: Modeling and GFP Imaging
A LAC SWITCH MODEL
We used a previously published synthetic switch, developed by Ceroni et al., to understand how our system could potentially be modeled and simulated.
AN INTERACTIVE MODEL
We used a model of the natural Lac operon to understand how changing the parameter values changes the behavior of the system.
COLLECTING IMPERICAL VALUES TO IMPROVE THE MODEL
We explored how one technique, imaging via microscopy could be used to determine the production rate of an output protein, in this case GFP in yeast, could be used to determine a "real" value for maximum GFP production rate under our own laboratory conditions.
Ideally, the GFP production rate measured by this method could be entered as a value for [which parameter] in the Ceroni et al. model.
Human Practices
Danger of Chemicals in Farmlands
Our Team
Your Name
My name is ###, and I am a ### majoring in ###. I am taking BME 494 because ###. An interesting fact about me is that ###.
Your Name
My name is ###, and I am a ### majoring in ###. I am taking BME 494 because ###. An interesting fact about me is that ###.
Your Name
My name is ###, and I am a ### majoring in ###. I am taking BME 494 because ###. An interesting fact about me is that ###.
Your Name
My name is ###, and I am a ### majoring in ###. I am taking BME 494 because ###. An interesting fact about me is that ###.
