BME494s2013 Project Team3: Difference between revisions
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By modifying the input and output for the Lac switch, it may be possible to produce materials such as plastics. The switch could be triggered by another environmental factor other than [IPTG], and instead of producing GFP, more useful materials like plastic could be an output. Currently, production of plastics is a very energy intensive process, and by using bacteria for the production, we can save energy and limit waste into the environment. | By modifying the input and output for the Lac switch, it may be possible to produce materials such as plastics. The switch could be triggered by another environmental factor other than [IPTG], and instead of producing GFP, more useful materials like plastic could be an output. Currently, production of plastics is a very energy intensive process, and by using bacteria for the production, we can save energy and limit waste into the environment. | ||
<!-- In the next paragraph, explain how the IPTG-input/ fluorescent protein-output Lac switch you proposed to build (in Unit 2) serves as a roof-of-concept for the practical application you just described --> | <!-- In the next paragraph, explain how the IPTG-input/ fluorescent protein-output Lac switch you proposed to build (in Unit 2) serves as a roof-of-concept for the practical application you just described --> | ||
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The IPTG-input/fluorescent protein-output is proof of concept for the production of synthetic materials such as plastic being created by bacteria. In the original project, a synthetic compound was utilized to trigger the metabolic pathway for the degradation of lactose. Using synthetic biology, we can splice together different genetic features to create an entirely new metabolic response. We can find a promoter that responds to a different input. In the natural Lac-operon, the cell produces proteins for the breakdown of lactose. We modified the natural process by initiating the production of GFP instead of the functional proteins found in the original process. It seems, that if we are able to control our protein output, we can produce synthetic products as well. | The IPTG-input/fluorescent protein-output is proof of concept for the production of synthetic materials such as plastic being created by bacteria. In the original project, a synthetic compound was utilized to trigger the metabolic pathway for the degradation of lactose. Using synthetic biology, we can splice together different genetic features to create an entirely new metabolic response. We can find a promoter that responds to a different input. In the natural Lac-operon, the cell produces proteins for the breakdown of lactose. We modified the natural process by initiating the production of GFP instead of the functional proteins found in the original process. It seems, that if we are able to control our protein output, we can produce synthetic products as well. | ||
Revision as of 12:29, 24 April 2013
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Overview & PurposeBy modifying the input and output for the Lac switch, it may be possible to produce materials such as plastics. The switch could be triggered by another environmental factor other than [IPTG], and instead of producing GFP, more useful materials like plastic could be an output. Currently, production of plastics is a very energy intensive process, and by using bacteria for the production, we can save energy and limit waste into the environment.
Background
Design: Our genetic circuitOUR GENE SWITCH:
Building: Assembly Scheme
Testing: Modeling and GFP Imaging
Human Practices
Our Team
Works Cited[1] Full reference. [2] Full reference. [3] Full reference.
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