BME494s2013 Project Team3

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(Background)
(Design: Our genetic circuit)
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[[Image:Circuit_diagramTEAM3.jpg|thumb|350px||left|Text describing the image]]
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[[Image:Circuit_diagramTEAM3.jpg|thumb|350px||left|AND gate Gene Switch: Both [IPTG] AND [Low Glucose Levels] conditions must be met for GFP production]]

Revision as of 19:15, 22 April 2013


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Contents

Overview & Purpose

Text describing the image
Text describing the image












Background

Diagram of Lac operon and how it functions without lactose present (top) and with lactose present (bottom)
Diagram of Lac operon and how it functions without lactose present (top) and with lactose present (bottom)














Design: Our genetic circuit

OUR GENE SWITCH:


AND gate Gene Switch: Both [IPTG] AND [Low Glucose Levels] conditions must be met for GFP production
AND gate Gene Switch: Both [IPTG] AND [Low Glucose Levels] conditions must be met for GFP production












Building: Assembly Scheme













Testing: Modeling and GFP Imaging


A LAC SWITCH MODEL
We used a previously published synthetic switch, developed by Ceroni et al., to understand how our system could potentially be modeled and simulated.


AN INTERACTIVE MODEL
We used a model of the natural Lac operon to understand how changing the parameter values changes the behavior of the system.


COLLECTING IMPERICAL VALUES TO IMPROVE THE MODEL
We explored how one technique, imaging via microscopy could be used to determine the production rate of an output protein, in this case GFP in yeast, could be used to determine a "real" value for maximum GFP production rate under our own laboratory conditions.



Ideally, the GFP production rate measured by this method could be entered as a value for [which parameter] in the Ceroni et al. model.











Human Practices

Danger of Chemicals in Farmlands
Danger of Chemicals in Farmlands














Our Team

Your Name
Your Name


  • My name is ###, and I am a ### majoring in ###. I am taking BME 494 because ###. An interesting fact about me is that ###.



Your Name
Your Name


  • My name is ###, and I am a ### majoring in ###. I am taking BME 494 because ###. An interesting fact about me is that ###.



Your Name
Your Name


  • My name is ###, and I am a ### majoring in ###. I am taking BME 494 because ###. An interesting fact about me is that ###.



Your Name
Your Name


  • My name is ###, and I am a ### majoring in ###. I am taking BME 494 because ###. An interesting fact about me is that ###.






Works Cited

[1] Full reference.

[2] Full reference.

[3] Full reference.


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