BME494s2013 Project Team1

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[[BME494_s2013:WikiHelp | <font face="trebuchet ms" style="color: #808080"> '''Wiki Editing Help''' </font>]]  
[[BME494_s2013:WikiHelp | <font face="trebuchet ms" style="color: #808080"> '''Wiki Editing Help''' </font>]]  
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==Overview & Purpose==
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<!-- Below is the code for making a thumbnail image to embed in your text. See BME494_Wiki_Help for help with uploading images to OpenWetWare and using them on your page.-->
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[[Image:CirEmblemHandwriteLogo2.png|thumb|140px||left|Text describing the image]]
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<!-- This is where you can get creative. Think of a practical application for a genetic input/ output switch that is based on the Lac switch you designed. Perhaps it can generate some useful compound instead of RFP/ GFP? Perhaps you could replace the Lac Repressor/ promoter part with something that binds to a different compound to make a sensor? Type a "10,000 foot" overview of your project below this comment line. What is your project's impact on the world? -->
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<!-- In the next paragraph, explain how the IPTG-input/ fluorescent protein-output Lac switch you proposed to build (in Unit 2) serves as a roof-of-concept for the practical application you just described -->
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<br><br><br><br><br><br><br><br>
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<!-- These are lines breaks for spacing purposes. You can add or delete these as needed -->
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==Background==
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[[Image:CirEmblemHandwriteLogo2.png|thumb|140px||left|Text describing the image]]
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<!-- Background information on the natural Lac operon. This should be based on Group Presentation 2 -->
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<br><br><br><br><br><br><br><br><br><br><br><br>
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<!-- These are lines breaks for spacing purposes. You can add or delete these as needed -->
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==Design: Our genetic circuit==
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<!--Delete the line items that do not apply to your project -->
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'''OUR GENE SWITCH''': 
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<!-- Show a network/ circuit diagram of your team's Lac switch. Include a paragraph to explain how it works (i.e., how to switch the system from on to off and vice versa, and what happens to each component as the system switches between states) -->
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[[Image:CirEmblemHandwriteLogo2.png|300px|Text describing the image]]
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<br><br><br><br><br><br><br><br><br>
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<!-- These are lines breaks for spacing purposes. You can add or delete these as needed -->
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==Building: Assembly Scheme==
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<!-- Illustrate and describe how you will build your lac switch. Incorporate information from Group Presentation 2 -->
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<br><br><br><br><br><br><br><br><br><br><br><br>
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<!-- These are lines breaks for spacing purposes. You can add or delete these as needed -->
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==Testing: Modeling and GFP Imaging==
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<br>
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'''A LAC SWITCH MODEL'''
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<br>
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We used a previously published synthetic switch, developed by Ceroni et al., to understand how our system could potentially be modeled and simulated.
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<!-- Continue this paragraph by explaining to a non-specialist what a mathematical model is and what parameter values are, in general. Include your network diagram illustration of the Ceroni et al. model and list all of the parameters you were able to map onto the model -->
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'''AN INTERACTIVE MODEL'''
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<br>
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We used a model of the natural Lac operon to understand how changing the parameter values changes the behavior of the system.
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<!-- Continue this paragraph by explaining how you interacted with the MatLab model. Include two or more images showing different output curves that were generated when you altered the IPTG concentration -->
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'''COLLECTING IMPERICAL VALUES TO IMPROVE THE MODEL'''
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<br>
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We explored how one technique, imaging via microscopy could be used to determine the production rate of an output protein, in this case GFP in yeast, could be used to determine a "real" value for maximum GFP production rate under our own laboratory conditions.
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<!-- Describe summarize how you measured GFP intensities, plotted charts in MatLab, make a curve of best fit, and tried to determine the maximum rate of GFP production. You don't have to include your raw values, just the graphs and equations. You may include some small images of the GFP-expressing yeast. If you didn't get a nice peak, explain how your image analysis might be changed to improve your outcome. -->
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<br>
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Ideally, the GFP production rate measured by this method could be entered as a value for [which parameter] in the Ceroni et al. model.
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<br><br><br><br><br><br><br><br>
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==Human Practices==
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[[Image:cow.jpg|thumb|130px|right|'''Danger of Chemicals in Farmlands''']]
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<!--Wait until Unit 3 to fill this in. Demonstrate that you have considered the societal aspects of your project - what could go wrong? Is it implementable? Etc.-->
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<br><br><br><br><br><br><br><br><br><br><br><br>
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==Our Team==
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<!-- Replace BME194_profile.jpg with your picture. Replace the "Your area of study..." with your own text. -->
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<div style="color: #808080; background-color: #ffffff; width: 600px; padding: 5px">
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[[Image:BME194_profile.jpg |thumb|noframe|130px|left|'''Your Name''']]
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* My name is ###, and I am a ### majoring in ###. I am taking BME 494 because ###. An interesting fact about me is that ###.
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</div>
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<br>
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<div style="color: #808080; background-color: #ffffff; width: 600px; padding: 5px">
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[[Image:BME194_profile.jpg |thumb|noframe|130px|left|'''Your Name''']]
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* My name is ###, and I am a ### majoring in ###. I am taking BME 494 because ###. An interesting fact about me is that ###.
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</div>
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<br>
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<div style="color: #808080; background-color: #ffffff; width: 600px; padding: 5px">
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[[Image:BME194_profile.jpg |thumb|noframe|130px|left|'''Your Name''']]
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* My name is ###, and I am a ### majoring in ###. I am taking BME 494 because ###. An interesting fact about me is that ###.
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</div>
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<br>
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<div style="color: #808080; background-color: #ffffff; width: 600px; padding: 5px">
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[[Image:BME194_profile.jpg |thumb|noframe|130px|left|'''Your Name''']]
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* My name is ###, and I am a ### majoring in ###. I am taking BME 494 because ###. An interesting fact about me is that ###.
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</div>
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<br>
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<br><br><br>
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==Works Cited==
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[1] Full reference.
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[2] Full reference.
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[3] Full reference.

Revision as of 18:16, 22 April 2013


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Contents

Overview & Purpose

Text describing the image
Text describing the image












Background

Text describing the image
Text describing the image














Design: Our genetic circuit

OUR GENE SWITCH:


Text describing the image












Building: Assembly Scheme













Testing: Modeling and GFP Imaging


A LAC SWITCH MODEL
We used a previously published synthetic switch, developed by Ceroni et al., to understand how our system could potentially be modeled and simulated.


AN INTERACTIVE MODEL
We used a model of the natural Lac operon to understand how changing the parameter values changes the behavior of the system.


COLLECTING IMPERICAL VALUES TO IMPROVE THE MODEL
We explored how one technique, imaging via microscopy could be used to determine the production rate of an output protein, in this case GFP in yeast, could be used to determine a "real" value for maximum GFP production rate under our own laboratory conditions.



Ideally, the GFP production rate measured by this method could be entered as a value for [which parameter] in the Ceroni et al. model.











Human Practices

Danger of Chemicals in Farmlands
Danger of Chemicals in Farmlands














Our Team

Your Name
Your Name


  • My name is ###, and I am a ### majoring in ###. I am taking BME 494 because ###. An interesting fact about me is that ###.



Your Name
Your Name


  • My name is ###, and I am a ### majoring in ###. I am taking BME 494 because ###. An interesting fact about me is that ###.



Your Name
Your Name


  • My name is ###, and I am a ### majoring in ###. I am taking BME 494 because ###. An interesting fact about me is that ###.



Your Name
Your Name


  • My name is ###, and I am a ### majoring in ###. I am taking BME 494 because ###. An interesting fact about me is that ###.






Works Cited

[1] Full reference.

[2] Full reference.

[3] Full reference.