BME494s2013 Project Team1: Difference between revisions
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The lac operon itself is a set of genes found in certain bacterias' DNA that is required for the transport and metabolism of lactose. Most commonly found in Escherichia coli, the operon was the first example of a group of genes under the control of an operator region to which a lactose repressor binds. | The lac operon itself is a set of genes found in certain bacterias' DNA that is required for the transport and metabolism of lactose. Most commonly found in Escherichia coli, the operon was the first example of a group of genes under the control of an operator region to which a lactose repressor binds. | ||
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The lac operon functions as a single transcription unit and is comprised of an operator, a promoter, and on or more structural genes such as a regulator or terminator that are transcribed into one polycistronic mRNA. When the bacteria are transferred to lactose-containing medium, allolactose (which forms when lactose is present in the cell) binds to the repressor, inhibits the binding of the repressor to the operator, and allows transcription of mRNA for enzymes involved in lactose metabolism and transport across the membrane. | The lac operon functions as a single transcription unit and is comprised of an operator, a promoter, and on or more structural genes such as a regulator or terminator that are transcribed into one polycistronic mRNA. When the bacteria are transferred to lactose-containing medium, allolactose (which forms when lactose is present in the cell) binds to the repressor, inhibits the binding of the repressor to the operator, and allows transcription of mRNA for enzymes involved in lactose metabolism and transport across the membrane. | ||
Revision as of 16:15, 22 April 2013
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Building a Sweet Cyan
Overview & PurposeSarah
Background
Design: Our genetic circuitJulia OUR GENE SWITCH: THE PARTS <tab>pSB1A3-1 is a high copy number plasmid. The replication origin is a pUC19-derived pMB1 (copy number of 100-300 per cell). The terminators bracketing pSB1A3 MCS are designed to prevent transcription from inside the MCS from reading out into the vector.
Building: Assembly SchemeEmily
Testing: Modeling and GFP Imaging
Human Practices
Our Team
Works Cited[1] Full reference. [2] Full reference. [3] Full reference.
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