BME494s2013 Project Team1: Difference between revisions
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'''Building a Sweet Cyan<br> | |||
Below is the content for our final project for the SPring 2013 Introduction to Synthetic BIology Class at Arizona State University. | |||
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==Overview & Purpose== | ==Overview & Purpose== | ||
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<!-- This is where you can get creative. Think of a practical application for a genetic input/ output switch that is based on the Lac switch you designed. Perhaps it can generate some useful compound instead of RFP/ GFP? Perhaps you could replace the Lac Repressor/ promoter part with something that binds to a different compound to make a sensor? Type a "10,000 foot" overview of your project below this comment line. What is your project's impact on the world? --> | <!-- This is where you can get creative. Think of a practical application for a genetic input/ output switch that is based on the Lac switch you designed. Perhaps it can generate some useful compound instead of RFP/ GFP? Perhaps you could replace the Lac Repressor/ promoter part with something that binds to a different compound to make a sensor? Type a "10,000 foot" overview of your project below this comment line. What is your project's impact on the world? --> | ||
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<!-- In the next paragraph, explain how the IPTG-input/ fluorescent protein-output Lac switch you proposed to build (in Unit 2) serves as a roof-of-concept for the practical application you just described --> | <!-- In the next paragraph, explain how the IPTG-input/ fluorescent protein-output Lac switch you proposed to build (in Unit 2) serves as a roof-of-concept for the practical application you just described --> | ||
Sarah | Sarah | ||
* What this can be used for | |||
* Sensing something | |||
[[Image:LacOperon.jpg|thumb|1040px||center|Basic Components of a Lac Operon]] | |||
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==Background== | ==Background== | ||
[[Image: | [[Image:NLacOperon.jpg|thumb|140px||left|Natural Lac Operon Parts/DNA Schematic]] | ||
<!-- Background information on the natural Lac operon. This should be based on Group Presentation 2 --> | <!-- Background information on the natural Lac operon. This should be based on Group Presentation 2 --> | ||
The lac operon itself is a set of genes found in certain bacterias' DNA that is required for the transport and metabolism of lactose. Most commonly found in Escherichia coli, the operon was the first example of a group of genes under the control of an operator region to which a lactose repressor binds. | |||
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The lac operon functions as a single transcription unit and is comprised of an operator, a promoter, and on or more structural genes such as a regulator or terminator that are transcribed into one polycistronic mRNA. When the bacteria are transferred to lactose-containing medium, allolactose (which forms when lactose is present in the cell) binds to the repressor, inhibits the binding of the repressor to the operator, and allows transcription of mRNA for enzymes involved in lactose metabolism and transport across the membrane. | |||
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'''OUR GENE SWITCH''': | '''OUR GENE SWITCH''': | ||
THE PARTS | |||
<tab>pSB1A3-1 is a high copy number plasmid. The replication origin is a pUC19-derived pMB1 (copy number of 100-300 per cell). The terminators bracketing pSB1A3 MCS are designed to prevent transcription from inside the MCS from reading out into the vector. | |||
<!-- Show a network/ circuit diagram of your team's Lac switch. Include a paragraph to explain how it works (i.e., how to switch the system from on to off and vice versa, and what happens to each component as the system switches between states) --> | <!-- Show a network/ circuit diagram of your team's Lac switch. Include a paragraph to explain how it works (i.e., how to switch the system from on to off and vice versa, and what happens to each component as the system switches between states) --> | ||
[[Image: | [[Image:Sweet_Cyan_Plasmid_Map.png|300px|Plasmid Map of "Sweet Cyan"]] | ||
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* My name is Julia Smith, and I am a senior majoring in Biomedical Engineering. I am taking BME 494 because I am extremely interested in synthetic biology. An interesting fact about me is that in addition to my nerdy side and love of accademic learning, I train reining horses. | * My name is Julia Smith, and I am a senior majoring in Biomedical Engineering. I am taking BME 494 because I am extremely interested in synthetic biology. An interesting fact about me is that in addition to my nerdy side and love of accademic learning, I train reining horses. | ||
Revision as of 16:15, 22 April 2013
Home People Course Projects Course Materials Schedule Photos Wiki Editing Help
Building a Sweet Cyan
Overview & PurposeSarah
Background
Design: Our genetic circuitJulia OUR GENE SWITCH: THE PARTS <tab>pSB1A3-1 is a high copy number plasmid. The replication origin is a pUC19-derived pMB1 (copy number of 100-300 per cell). The terminators bracketing pSB1A3 MCS are designed to prevent transcription from inside the MCS from reading out into the vector.
Building: Assembly SchemeEmily
Testing: Modeling and GFP Imaging
Human Practices
Our Team
Works Cited[1] Full reference. [2] Full reference. [3] Full reference.
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