BME494 Sp2014 Tran: Difference between revisions
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==Background & Proposed Application== | ==Background & Proposed Application== | ||
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The malarial biosensor is modeled after the classical synthetic toggle switch developed in Escherichia coli, or E. Coli (Gardner et al 2000). The precedent synthetic system was developed with two different inducible states, an “on” and “off” state. The system was created using two promoter and repressor pairs. Both repressors were inhibited by a different inducer (aTc and IPTG). The promoters were placed upstream of the repressor gene of the opposite pair. This created a bistable system where only one promoter would be expressed at any one time, since the expression of a promoter repressed expression of the other promoter. To distinguish a cell between its on state and off state, a green fluorescence protein transcription gene was placed downstream of the on state promoter so the cell would exhibit green fluorescence in its on state. | |||
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Revision as of 22:06, 21 April 2014
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Background & Proposed ApplicationBACKGROUND
The malarial biosensor is modeled after the classical synthetic toggle switch developed in Escherichia coli, or E. Coli (Gardner et al 2000). The precedent synthetic system was developed with two different inducible states, an “on” and “off” state. The system was created using two promoter and repressor pairs. Both repressors were inhibited by a different inducer (aTc and IPTG). The promoters were placed upstream of the repressor gene of the opposite pair. This created a bistable system where only one promoter would be expressed at any one time, since the expression of a promoter repressed expression of the other promoter. To distinguish a cell between its on state and off state, a green fluorescence protein transcription gene was placed downstream of the on state promoter so the cell would exhibit green fluorescence in its on state. 
Design of a New Device
Building the New DeviceSYNTHETIC DNA LAYOUT
Testing the New DeviceLAC OPERON MODEL SIMULATION
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