BME494 Sp2014 Dhatt

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Background & Proposed Application BACKGROUND The synthetic system modeled in the “Construction of a genetic toggle switch In Escherichia coli” uses two different states, an “on” state” and an “off” state (Gardner et all 2000). The classical system uses two repressor and two promoter pairs in which both repressors are inhibited by a different inducer, aTc and IPTG. The promoters were placed next to the repressor gene of the opposite pair. This system represents a bistable gene-regulatory network where only one promoter can be expressed at one time because the expression of one promoter repressed expression of the other promoter. The on-state of the cell was represented by placing a green fluorescent protein (GFP) transcription gene downstream of the on-state promoter. Classical Toggle Switch Design (Gardner et al, 2000) APPLICATION OF MY PROPOSED NEW DEVICE Design of a New Device Building the New Device SYNTHETIC DNA LAYOUT RESOURCES TYPE IIS ASSEMBLY Testing the New Device LAC OPERON MODEL SIMULATION I used a model of the natural Lac operon to learn how changing the parameter values changes the behavior of the system. RELATIONSHIP BETWEEN THE LAC MODEL AND MY NEW DESIGN Similarities: Differences: TESTING THE NEW DEVICE Human Practices Danger of Chemicals in Farmlands About the Designer Your Name My name is ###, and I am a ### majoring in ###. I am taking BME 494 because ###. An interesting fact about me is that ###. References [1] Full reference. [2] Full reference. [3] Full reference.