BME494 Sp2014 Dhatt: Difference between revisions
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<!-- Incorporate information from Presentation 1. Summarize the classical synthetic device upon which your new design is based. If you post an image of a figure from a paper, include an in-text citation in the text legend. --> | <!-- Incorporate information from Presentation 1. Summarize the classical synthetic device upon which your new design is based. If you post an image of a figure from a paper, include an in-text citation in the text legend. --> | ||
The synthetic system modeled in the “Construction of a genetic toggle switch In Escherichia coli” uses two different states, an “on” state” and an “off” state (Gardner et all 2000). The classical system uses two repressor and two promoter pairs in which both repressors are inhibited by a different inducer, aTc and IPTG. The promoters were placed next to the repressor gene of the opposite pair. This system represents a bistable gene-regulatory network where only one promoter can be expressed at one time because the expression of one promoter repressed expression of the other promoter. The on-state of the cell was represented by placing a green fluorescent protein (GFP) transcription gene downstream of the on-state promoter. | |||
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[[Image: | [[Image:BME494_Dhatt_ClassicalDesignGardner.jpg|thumb|140px||left|Classical Toggle Switch Design (Gardner et al, 2000)]] | ||
Revision as of 13:10, 8 May 2014
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Background & Proposed ApplicationBACKGROUND
The synthetic system modeled in the “Construction of a genetic toggle switch In Escherichia coli” uses two different states, an “on” state” and an “off” state (Gardner et all 2000). The classical system uses two repressor and two promoter pairs in which both repressors are inhibited by a different inducer, aTc and IPTG. The promoters were placed next to the repressor gene of the opposite pair. This system represents a bistable gene-regulatory network where only one promoter can be expressed at one time because the expression of one promoter repressed expression of the other promoter. The on-state of the cell was represented by placing a green fluorescent protein (GFP) transcription gene downstream of the on-state promoter. 
Design of a New Device
Building the New DeviceSYNTHETIC DNA LAYOUT
Testing the New DeviceLAC OPERON MODEL SIMULATION
Similarities:
Differences:
Human Practices
About the Designer
References[1] Full reference. [2] Full reference. [3] Full reference.
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