BME103 s2013:TEMPwu3

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Lab Write-Up 1
Lab Write-Up 2
Lab Write-Up 3
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Contents

OUR TEAM

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LAB 3 WRITE-UP

Original System: PCR Results

PCR Test Results

Sample Name ng/mL Conclusion (pos/neg)
Positive Control --- N/A
Negative Control --- N/A
Tube Label:___ Patient ID: ____ rep 1 ---
Tube Label:___ Patient ID: ____ rep 2 ---
Tube Label:___ Patient ID: ____ rep 3 ---
Tube Label:___ Patient ID: ____ rep 1 ---
Tube Label:___ Patient ID: ____ rep 2 ---
Tube Label:___ Patient ID: ____ rep 3 ---


Bayesian Statistics
These statistics are based upon all of the DNA detection system results that were obtained in the PCR lab for 34 hypothetical patients who were diagnosed as either having cancer or not having cancer.

Bayes Theorem equation: P(A|B) = P(B|A) * P(A) / P(B)


Calculation 1: The probability that the sample actually has the cancer DNA sequence, given a positive diagnostic signal.

  • A = [text description] = [frequency shown as a fraction] = [final numerical value]
  • B = [text description] = [frequency shown as a fraction] = [final numerical value]
  • P (B|A) = [text description] = [frequency shown as a fraction] = [final numerical value]
  • P(A|B) = [answer]



Calculation 3: The probability that the patient will develop cancer, given a cancer DNA sequence.

  • A = [text description] = [frequency shown as a fraction] = [final numerical value]
  • B = [text description] = [frequency shown as a fraction] = [final numerical value]
  • P (B|A) = [text description] = [frequency shown as a fraction] = [final numerical value]
  • P(A|B) = [answer]



New System: Design Strategy

We concluded that a good system Must Have:

  • [Must have #1 - why? short, ~4 or 5 sentences]
  • [Must have #2 - why? short, ~4 or 5 sentences]


We concluded that we would Want a good system to have:

  • [Want #1 - why? short, ~4 or 5 sentences]
  • [Want #2 - why? short, ~4 or 5 sentences


We concluded that a good system Must Not Have:

  • [Must Not Have #1 - why? short, ~4 or 5 sentences]
  • [Must Not Have #2 - why? short, ~4 or 5 sentences]


We concluded that a good system Should Avoid:

  • [Should Avoid #1 - why? short, ~4 or 5 sentences]
  • [Should Avoid #2 - why? short, ~4 or 5 sentences]




New System: Machine/ Device Engineering

DESIGN STRATEGY


We chose to include these new features

  • Feature 1 - explanation of how this addresses any of the the Must Have/ Must Not Have items in the
  • Feature 2 - description * Etc.


[OR]


We chose keep the devices the same as the original system
[Short paragraph explaining why the original system satisfies the design needs that you listed above]


DESIGN APPROACH




Protocols

Materials


PCR Protocol



DNA Measurement Protocol



Research and Development

Background on Disease Markers



Primer Design



Illustration




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