BME103 s2013:TEMPwu3
OUR TEAM
LAB 2 WRITE-UP
Background Information
SYBR Green Dye
[A short summary describing SYBR green dye]
Single-Drop Fluorimeter
[A description of the single-drop fluorimeter device. Add a PHOTO for bonus points]
How the Fluorescence Technique Works
[In your own words, a summary of the information from page 9 of the worksheet]
Procedure
Smart Phone Camera Settings
- [The type of smart phone you used and how you adjusted the camera settings, if applicable (see worksheet page 4).]
- Flash:
- ISO setting:
- White Balance:
- Exposure:
- Saturation:
- Contrast:
- [If you used an additional phone, describe the other type of smart phone you used and how you adjusted the camera settings, if applicable (see worksheet page 4).]
- Flash:
- ISO setting:
- White Balance:
- Exposure:
- Saturation:
- Contrast:
Calibration
[Describe how to set up your camera in front of the fluorimeter. Add a PHOTO of this set-up for bonus points.]
- Distance between the smart phone cradle and drop =
[See worksheet page 5]
Solutions Used for Calibration [See worksheet page 5]
row 1 cell 1 | row 1 cell 2 | row 1 cell 3 | row 1 cell 4 |
row 2 cell 1 | row 2 cell 2 | row 2 cell 3 | row 2 cell 4 |
row 3 cell 1 | row 3 cell 2 | row 3 cell 3 | row 3 cell 4 |
[Add more rows as needed]
Placing Samples onto the Fluorimeter
- [Step one, in your OWN words]
- [Step two, in your own words]
- [Step three, in your own words]
- [etc.]
Data Analysis
Representative Images of Samples
[Show an IMAGE where you drew a circle around the droplet with the freehand tool for a sample with no DNA]
[Show an IMAGE where you drew a circle around the droplet with the freehand tool for a sample with DNA (positive signal)]
Image J Values for All Samples [See worksheet page 5]
row 1 cell 1 | row 1 cell 2 | row 1 cell 3 | row 1 cell 4 | row 1 cell 5 |
row 2 cell 1 | row 2 cell 2 | row 2 cell 3 | row 2 cell 4 | row 2 cell 5 |
row 3 cell 1 | row 3 cell 2 | row 3 cell 3 | row 3 cell 4 | row 2 cell 5 |
[Add more rows as needed]
Fitting a Straight Line
[Place an IMAGE of your Excel plot with a line of best fit here. See worksheet page 8]