BME103 s2013:TEMPwu3: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> BME 103 Spring 2013</span>
|style="background-color: #F2F2F2" | [[BME103_s2013 | <font face="trebuchet ms" style="color: #808080"> '''Home''' </font>]]<br>[[BME103_s2013:People | <font face="trebuchet ms" style="color: #808080"> '''People''' </font>]]<br>[[BME103_s2013:Projects | <font face="trebuchet ms" style="color: #808080"> '''Lab Write-Up 1''' </font>]]<br>[[BME103_s2013:Projects2 | <font face="trebuchet ms" style="color: #808080"> '''Lab Write-Up 2''' </font>]]<br>[[BME103_s2013:Projects3 | <font face="trebuchet ms" style="color: #808080"> '''Lab Write-Up 3''' </font>]]<br>[[BME103_s2013:Logistics | <font face="trebuchet ms" style="color: #808080"> ''' Course Logistics For Instructors''' </font>]] <br>[[BME103_s2013:Photos | <font face="trebuchet ms" style="color: #808080"> '''Photos''' </font>]] <br>[[BME103_s2013:WikiHelp | <font face="trebuchet ms" style="color: #808080"> '''Wiki Editing Help''' </font>]]
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=OUR TEAM=
=OUR TEAM=



Revision as of 14:50, 2 April 2013

BME 103 Spring 2013 Home
People
Lab Write-Up 1
Lab Write-Up 2
Lab Write-Up 3
Course Logistics For Instructors
Photos
Wiki Editing Help

OUR TEAM

Name: student
Name: student
Name: student
Name: student
Name: student
Name: student


LAB 2 WRITE-UP

Background Information

SYBR Green Dye
[A short summary describing SYBR green dye]


Single-Drop Fluorimeter
[A description of the single-drop fluorimeter device. Add a PHOTO for bonus points]


How the Fluorescence Technique Works
[In your own words, a summary of the information from page 9 of the worksheet]



Procedure

Smart Phone Camera Settings

  • [The type of smart phone you used and how you adjusted the camera settings, if applicable (see worksheet page 4).]
    • Flash:
    • ISO setting:
    • White Balance:
    • Exposure:
    • Saturation:
    • Contrast:
  • [If you used an additional phone, describe the other type of smart phone you used and how you adjusted the camera settings, if applicable (see worksheet page 4).]
    • Flash:
    • ISO setting:
    • White Balance:
    • Exposure:
    • Saturation:
    • Contrast:


Calibration

[Describe how to set up your camera in front of the fluorimeter. Add a PHOTO of this set-up for bonus points.]

  • Distance between the smart phone cradle and drop =

[See worksheet page 5]


Solutions Used for Calibration [See worksheet page 5]

row 1 cell 1 row 1 cell 2 row 1 cell 3 row 1 cell 4
row 2 cell 1 row 2 cell 2 row 2 cell 3 row 2 cell 4
row 3 cell 1 row 3 cell 2 row 3 cell 3 row 3 cell 4

[Add more rows as needed]


Placing Samples onto the Fluorimeter

  • [Step one, in your OWN words]
  • [Step two, in your own words]
  • [Step three, in your own words]
  • [etc.]


Data Analysis

Representative Images of Samples

[Show an IMAGE where you drew a circle around the droplet with the freehand tool for a sample with no DNA]

[Show an IMAGE where you drew a circle around the droplet with the freehand tool for a sample with DNA (positive signal)]


Image J Values for All Samples [See worksheet page 5]

row 1 cell 1 row 1 cell 2 row 1 cell 3 row 1 cell 4 row 1 cell 5
row 2 cell 1 row 2 cell 2 row 2 cell 3 row 2 cell 4 row 2 cell 5
row 3 cell 1 row 3 cell 2 row 3 cell 3 row 3 cell 4 row 2 cell 5

[Add more rows as needed]


Fitting a Straight Line
[Place an IMAGE of your Excel plot with a line of best fit here. See worksheet page 8]