BME103 s2013:TEMPwu3: Difference between revisions
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<br><br> | <br><br> | ||
==New System: Protocols== | ==New System: Protocols== | ||
'''DESIGN AND MATERIALS''' | '''DESIGN AND MATERIALS''' | ||
<!-- | |||
<!-- If your team decided to change the PCR and/or the Fluorimeter imaging protocols, summarize the new approaches/ features here and delete the '''We chose keep the protocols the same as the original system''' section. --> | |||
'''We chose to include these new approaches/ features''' | |||
* Feature 1 - explanation of how this addresses any of the specifications in the "New System: Design Strategy" section | |||
* Feature 2 - explanation of how this addresses any of the specifications in the "New System: Design Strategy" section | |||
* Etc. | |||
If | |||
[OR] | |||
<!-- If your team decided NOT to change any of the machinery/ devices, explain why here and delete the '''We chose to include these new features''' section above--> | |||
'''We chose keep the protocols the same as the original system''' | |||
* Feature 1 - explanation of how a pre-existing feature addresses any of the specifications in the "New System: Design Strategy" section | |||
* Feature 2 - explanation of how a pre-existing feature addresses any of the specifications in the "New System: Design Strategy" section | |||
* Etc. | |||
'''MATERIALS''' | '''MATERIALS''' | ||
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''' | '''PROTOCOLS''' | ||
* '''PCR Protocol''' | |||
<!-- Create a step-by-step procedure for setting up and running PCR reactions. Your instructions should include everything from adding reagents to the tubes, to programming the PCR machine and running the reaction.--> | |||
* '''DNA Measurement and Analysis Protocol''' | |||
<!-- Create a step-by-step procedure for measuring DNA amplification in the PCR reactions. Your instructions should include everything from diluting the samples in SYBR Green, to placing the drops onto the fluorimeter (if your group is using the fluorimeter), to collecting and processing images in Image J. Don't forget to provide instructions on how to set up the calf thymus DNA samples for calibration, and how to convert INTDEN values into concentrations.---> | |||
==Research and Development== | ==New System: Research and Development== | ||
'''BACKGROUND''' | |||
<!--- A description of the CHEK2 gene, it's associated SNP, and the cancer-related function of the gene. Use the information from your Week 13 worksheet. ---> | |||
'''DESIGN''' | |||
<!-- If your team decided to only amplify cancer-associated DNA, list the Cancer allele forward primer and the Cancer allele reverse primer. Include a paragraph that explains why a disease allele will give a PCR product and the non-disease allele will not.--> | |||
<!-- If your team chose an alternative approach to amplify the DNA, list all relevant primers. Include a paragraph that explains how your system works.--> | |||
'''Our primers address the following design needs''' | |||
* Design specification 1 - explanation of how an aspect of the primers addresses any of the specifications in the "New System: Design Strategy" section | |||
* Design specification 2 - explanation of how an aspect of the primers addresses any of the specifications in the "New System: Design Strategy" section | |||
* Etc. | |||
Revision as of 18:01, 9 April 2013
BME 103 Spring 2013 | Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help | ||||||||||||||||||||||||||||||||
OUR TEAMLAB 3 WRITE-UPOriginal System: PCR ResultsPCR Test Results
Bayes Theorem equation: P(A|B) = P(B|A) * P(A) / P(B)
Calculation 3: The probability that the patient will develop cancer, given a cancer DNA sequence.
New System: Design StrategyWe concluded that a good system Must Have:
New System: Machine/ Device EngineeringSYSTEM DESIGN
KEY FEATURES We chose to include these new features
[OR] We chose keep the devices the same as the original system
INSTRUCTIONS
New System: ProtocolsDESIGN AND MATERIALS We chose to include these new approaches/ features
[OR] We chose keep the protocols the same as the original system
MATERIALS
PROTOCOLS
New System: Research and DevelopmentBACKGROUND
DESIGN
Our primers address the following design needs
|